Central Carbon Metabolism, Sodium-Motive Electron Transfer, and Ammonium Formation by the Vaginal Pathogen Prevotella bivia
Abstract
:1. Introduction
2. Results
2.1. CO2-Dependent Succinate Formation by P. bivia
2.2. Ammonia Formation from L-Asparagine by P. bivia
2.3. Membrane-Bound Electron Transfer Complexes in P. bivia
2.4. NADH:Quinone and Quinol:Fumarate Oxidoreduction Activities of P. bivia Membranes
2.5. Sodium Dependent Membrane Potential in P. bivia
2.6. Cytochrome bd Quinol Oxidase of P. bivia
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Growth Conditions
4.2. Isolation and Solubilization of Membranes
4.3. Bacterial Growth
4.4. Analytical Methods
4.5. UV/Vis Difference Spectra of Redox Cofactors in P. bivia
4.6. Enzymatic Assays
4.7. Membrane Potential
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Growth Condition | OD600 (t = 7 d) | Doubling Time (h) | NH4+ (mM; t = 0) | NH4+ (mM; t = 7) | Net (NH3 + NH4+) Formed (mM) | Rate of Net (NH3 + NH4+) Formation (nmol min−1 mg−1) |
---|---|---|---|---|---|---|
pH 5 + Asn | 0.5 ± 0.1 | 91 | 14.7 ± 0.2 | 62.9 ± 0.8 | 48.2 ± 4.4 | 265.7 ± 24.1 |
pH 5 − Asn | 0.5 ± 0.1 | 101 | 14.8 ± 0.1 | 27.9 ± 0.2 | 13.1 ± 0.1 | 59.7 ± 0.4 |
pH 6 + Asn | 0.4 ± 0.1 | 17 | 16.9 ± 2.1 | 92.4 ± 1.1 | 75.5 ± 5.6 | 416.4 ± 31.4 |
pH 6 − Asn | 1.1 ± 0.4 | 13 | 12.1 ± 0.5 | 25.5 ± 0.2 | 13.1 ± 0.6 | 21.7 ± 2.1 |
pH 7 + Asn | 1.6 ± 0.4 | 10 | 18.1 ± 0.8 | 101.5 ± 0.5 | 83.4 ± 4.1 | 119.1 ± 2.5 |
pH 7 − Asn | 1.8 ± 0.2 | 8 | 16.5 ± 0.7 | 37.6 ± 0.03 | 21.1 ± 0.25 | 25.2 ± 0.5 |
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Schleicher, L.; Herdan, S.; Fritz, G.; Trautmann, A.; Seifert, J.; Steuber, J. Central Carbon Metabolism, Sodium-Motive Electron Transfer, and Ammonium Formation by the Vaginal Pathogen Prevotella bivia. Int. J. Mol. Sci. 2021, 22, 11925. https://doi.org/10.3390/ijms222111925
Schleicher L, Herdan S, Fritz G, Trautmann A, Seifert J, Steuber J. Central Carbon Metabolism, Sodium-Motive Electron Transfer, and Ammonium Formation by the Vaginal Pathogen Prevotella bivia. International Journal of Molecular Sciences. 2021; 22(21):11925. https://doi.org/10.3390/ijms222111925
Chicago/Turabian StyleSchleicher, Lena, Sebastian Herdan, Günter Fritz, Andrej Trautmann, Jana Seifert, and Julia Steuber. 2021. "Central Carbon Metabolism, Sodium-Motive Electron Transfer, and Ammonium Formation by the Vaginal Pathogen Prevotella bivia" International Journal of Molecular Sciences 22, no. 21: 11925. https://doi.org/10.3390/ijms222111925