CD38 Correlates with an Immunosuppressive Treg Phenotype in Lupus-Prone Mice
Abstract
:1. Introduction
2. Results
2.1. CD38 Expression Positively Correlates with FoxP3 Expression
2.2. CD38 Expression Positively Correlates with Immunosuppressive Molecules
2.3. Reduced CD38+ Treg Proportion in B6.MRL-Faslpr/J Mice
2.4. Reduced Treg Expansion and IL-10 Secretion in Stimulated CD38-/- Splenocytes
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Isolation of Splenocytes
4.3. Flow Cytometry
4.4. Expansion Treg Assay
4.5. IFN-γ and IL-10 Detection
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Pérez-Lara, J.C.; Espinosa, E.; Santos-Argumedo, L.; Romero-Ramírez, H.; López-Herrera, G.; García-García, F.; Sandoval-Montes, C.; Ortiz-Navarrete, V.; Flores-Muñoz, M.; Rodríguez-Alba, J.C. CD38 Correlates with an Immunosuppressive Treg Phenotype in Lupus-Prone Mice. Int. J. Mol. Sci. 2021, 22, 11977. https://doi.org/10.3390/ijms222111977
Pérez-Lara JC, Espinosa E, Santos-Argumedo L, Romero-Ramírez H, López-Herrera G, García-García F, Sandoval-Montes C, Ortiz-Navarrete V, Flores-Muñoz M, Rodríguez-Alba JC. CD38 Correlates with an Immunosuppressive Treg Phenotype in Lupus-Prone Mice. International Journal of Molecular Sciences. 2021; 22(21):11977. https://doi.org/10.3390/ijms222111977
Chicago/Turabian StylePérez-Lara, Jocelyn C., Enrique Espinosa, Leopoldo Santos-Argumedo, Héctor Romero-Ramírez, Gabriela López-Herrera, Fabio García-García, Claudia Sandoval-Montes, Vianney Ortiz-Navarrete, Mónica Flores-Muñoz, and Juan C. Rodríguez-Alba. 2021. "CD38 Correlates with an Immunosuppressive Treg Phenotype in Lupus-Prone Mice" International Journal of Molecular Sciences 22, no. 21: 11977. https://doi.org/10.3390/ijms222111977