MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
Abstract
:1. Introduction
2. Results
2.1. GmMATE1 and GmMATE2 Are Isoflavone Transporters in Soybeans
2.2. Both GmMATE1 and GmMATE2 Were Localized in the Vacuolar Membrane
2.3. GmMATE1 and GmMATE2 Were Expressed in Developing Pods, Seeds, and the Seed Coat
2.4. Manipulation of GmMATE1 in Soybean Significantly Altered the Isoflavone Contents in Seeds
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Gene Coning, Vector Construction and Transformation
4.3. Yeast Uptake Assay
4.4. Extraction of Seed Isoflavones
4.5. Subcellular Localization of GmMATE1 and GmMATE2 in Tobacco BrightYellow-2 (BY-2) Cells
4.6. Expression of GmMATE1 and GmMATE2 in Pod, Seed, and Seed Coat
4.7. β-Glucuronidase (GUS) Staining
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ng, M.-S.; Ku, Y.-S.; Yung, W.-S.; Cheng, S.-S.; Man, C.-K.; Yang, L.; Song, S.; Chung, G.; Lam, H.-M. MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds. Int. J. Mol. Sci. 2021, 22, 12017. https://doi.org/10.3390/ijms222112017
Ng M-S, Ku Y-S, Yung W-S, Cheng S-S, Man C-K, Yang L, Song S, Chung G, Lam H-M. MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds. International Journal of Molecular Sciences. 2021; 22(21):12017. https://doi.org/10.3390/ijms222112017
Chicago/Turabian StyleNg, Ming-Sin, Yee-Shan Ku, Wai-Shing Yung, Sau-Shan Cheng, Chun-Kuen Man, Liu Yang, Shikui Song, Gyuhwa Chung, and Hon-Ming Lam. 2021. "MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds" International Journal of Molecular Sciences 22, no. 21: 12017. https://doi.org/10.3390/ijms222112017