The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome
Abstract
:1. Introduction
2. Results
2.1. The Bam Complex Localizes at Midcell during Constriction
2.2. All BAM Subunits Localize at Midcell
2.3. Not All OM Proteins Exhibit a Midcell Localization like BAM
2.4. The BAM Complex Localizes at Midcell Simultaneously with Divisome Maturation
2.5. The BAM Midcell Localization Does Not Require Active Septation
2.6. The BAM Midcell Localization Does Not Depend on the Late Division Proteins
2.7. The BAM Complex Midcell Localization Depends on the Z-Ring Formation
2.8. YidC Colocalize at Midcell at the Same Time as the BAM Complex
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Culture Conditions
4.2. Fixation and Immunolabelling
4.3. Microscopy
4.4. Image Analysis
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Genotype | Reference |
---|---|---|
BW25113 | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | [62] |
BW25113ΔompA | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 ompA::kan | [62] |
LMC500 | F- araD139 Δ(argF-lac)U169 deoC1 flbB5301 ptsF25 rbsR relA1 rpsL150 lysA1 | [63] |
ΔbamB | BW25113 ΔbamB::cam | [64] |
ΔbamC | BW25113 ΔbamC::cam | [64] |
ΔbamE | BW25113 ΔbamE::cam | [64] |
PBP3ts | LMC500 ftsI(ts) | [47] |
FtsQts | LMC500 ftsQ(ts) | [47] |
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Consoli, E.; Luirink, J.; den Blaauwen, T. The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome. Int. J. Mol. Sci. 2021, 22, 12101. https://doi.org/10.3390/ijms222212101
Consoli E, Luirink J, den Blaauwen T. The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome. International Journal of Molecular Sciences. 2021; 22(22):12101. https://doi.org/10.3390/ijms222212101
Chicago/Turabian StyleConsoli, Elisa, Joen Luirink, and Tanneke den Blaauwen. 2021. "The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome" International Journal of Molecular Sciences 22, no. 22: 12101. https://doi.org/10.3390/ijms222212101