Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth
Abstract
:1. Introduction
2. Results
2.1. Negative Effect of MMP14Sf−/− Fibroblast Matrix on Melanoma Proliferation and Migration
2.2. Altered Composition of MMP14Sf−/− Fibroblast ECM
2.3. Collagen XIV Inhibits Pro-Invasive Activities
2.4. Collagen XIV Is Accumulated in Melanoma Grown in Fibrotic Skin
2.5. Collagen XIV Expression in Human Tissue
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Migration: Colony Outgrow Assay
4.3. BrdU Incorporation Assay
4.4. In Vitro Cleavage Assay
4.5. Immunoblot
4.6. Preparation of Samples for Proteome Analysis
4.7. Tumor Grafting Experiments
4.8. Immunofluorescence Staining
4.9. Immunohistochemical Staining
4.10. RNA Isolation, RT-PCR, and Real-Time PCR
4.11. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pach, E.; Kümper, M.; Fromme, J.E.; Zamek, J.; Metzen, F.; Koch, M.; Mauch, C.; Zigrino, P. Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth. Int. J. Mol. Sci. 2021, 22, 12276. https://doi.org/10.3390/ijms222212276
Pach E, Kümper M, Fromme JE, Zamek J, Metzen F, Koch M, Mauch C, Zigrino P. Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth. International Journal of Molecular Sciences. 2021; 22(22):12276. https://doi.org/10.3390/ijms222212276
Chicago/Turabian StylePach, Elke, Maike Kümper, Julia E. Fromme, Jan Zamek, Fabian Metzen, Manuel Koch, Cornelia Mauch, and Paola Zigrino. 2021. "Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth" International Journal of Molecular Sciences 22, no. 22: 12276. https://doi.org/10.3390/ijms222212276