Genome-Wide Identification of the Xyloglucan endotransglucosylase/Hydrolase (XTH) and Polygalacturonase (PG) Genes and Characterization of Their Role in Fruit Softening of Sweet Cherry
Abstract
:1. Introduction
2. Results
2.1. Identification of XTH and PG Family Genes in Prunus avium
2.2. Phylogenetic Analysis and Multiple Sequence Alignments of the PavXTH and PavPG Gene Family Members
2.3. Gene Structure, Conserved Motif and Cis-Elements Analysis of PavXTHs and PavPGs
2.4. Identification of PavXTH and PavPG Genes Underlying Involved in Fruit Softening
2.5. Expression of PavXTH14, PavXTH15 and PavPG38 Gene Expression in Response to Hormones
2.6. Transient Overexpression PavXTH14, PavXTH15 and PavPG38 in Cherry Fruits
3. Discussion
3.1. Characteristics of PavXTH and PavPG Gene Families
3.2. Expression Profiles of PavXTH and PavPG Genes
3.3. PavXTH14, PavXTH15 and PavPG38 Participate in Softening by Disrupting the Fruit Cell Wall
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Mining of PavXTH and PavPG Genes in the Prunus Avium Genome
4.3. Phylogenetic Analysis, Multiple Sequence Alignment, Genomic Structure, Chromosomal Location, and Motif Analysis
4.4. Cis-Element Prediction in the PavXTH and PavPG Gene Promoters
4.5. Measurement of Fruit Firmness and Cell Wall Materials
4.6. RT-qPCR Analysis
4.7. Transient Overexpression in Cherry Fruits
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Branch | Gene Locus | Gene Name | Deduced Protein | Signal Peptide | ||
---|---|---|---|---|---|---|
Length (aa) | PI | MW (kDa) | ||||
Ⅰ | Pav_sc0000359.1_g040.1.mk | PavXTH1 | 150 | 6.34 | 17.43 | - |
Pav_sc0000308.1_g610.1.mk | PavXTH2 | 372 | 5.47 | 42.11 | + | |
Pav_sc0003915.1_g020.1.mk | PavXTH3 | 176 | 9.68 | 20.5 | - | |
Pav_sc0000428.1_g530.1.mk | PavXTH8 | 282 | 8.39 | 31.93 | + | |
Pav_sc0000428.1_g520.1.mk | PavXTH9 | 193 | 9.14 | 22.37 | - | |
Pav_sc0000428.1_g510.1.mk | PavXTH10 | 316 | 5.2 | 36.53 | - | |
Pav_sc0001289.1_g270.1.mk | PavXTH13 | 204 | 5.2 | 23.18 | + | |
Pav_sc0000212.1_g420.1.mk | PavXTH15 | 197 | 6.06 | 22.31 | + | |
Ⅱ | Pav_sc0001450.1_g080.1.mk | PavXTH4 | 211 | 7.2 | 25.2 | - |
Ⅲ | Pav_sc0000480.1_g990.1.mk | PavXTH14 | 293 | 6.22 | 32.95 | + |
Pav_sc0000558.1_g1030.1.mk | PavXTH17 | 639 | 6.44 | 73.43 | - | |
Ⅳ | Pav_sc0000405.1_g520.1.mk | PavXTH11 | 287 | 6.89 | 32.51 | + |
Pav_sc0000910.1_g790.1.mk | PavXTH12 | 212 | 4.92 | 24.4 | + | |
Pav_sc0003766.1_g160.1.mk | PavXTH16 | 277 | 9.41 | 32.62 | + | |
Pav_sc0006464.1_g040.1.mk | PavXTH18 | 152 | 7.9 | 17.49 | - | |
Ⅴ | Pav_sc0000354.1_g310.1.mk | PavXTH5 | 314 | 7.95 | 35.1 | + |
Pav_sc0000893.1_g780.1.mk | PavXTH6 | 168 | 4.98 | 19.01 | + | |
Pav_sc0001673.1_g050.1.mk | PavXTH7 | 215 | 5.34 | 24.16 | - |
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Zhai, Z.; Feng, C.; Wang, Y.; Sun, Y.; Peng, X.; Xiao, Y.; Zhang, X.; Zhou, X.; Jiao, J.; Wang, W.; et al. Genome-Wide Identification of the Xyloglucan endotransglucosylase/Hydrolase (XTH) and Polygalacturonase (PG) Genes and Characterization of Their Role in Fruit Softening of Sweet Cherry. Int. J. Mol. Sci. 2021, 22, 12331. https://doi.org/10.3390/ijms222212331
Zhai Z, Feng C, Wang Y, Sun Y, Peng X, Xiao Y, Zhang X, Zhou X, Jiao J, Wang W, et al. Genome-Wide Identification of the Xyloglucan endotransglucosylase/Hydrolase (XTH) and Polygalacturonase (PG) Genes and Characterization of Their Role in Fruit Softening of Sweet Cherry. International Journal of Molecular Sciences. 2021; 22(22):12331. https://doi.org/10.3390/ijms222212331
Chicago/Turabian StyleZhai, Zefeng, Chen Feng, Yanyan Wang, Yueting Sun, Xiang Peng, Yuqin Xiao, Xiang Zhang, Xin Zhou, Jiale Jiao, Weili Wang, and et al. 2021. "Genome-Wide Identification of the Xyloglucan endotransglucosylase/Hydrolase (XTH) and Polygalacturonase (PG) Genes and Characterization of Their Role in Fruit Softening of Sweet Cherry" International Journal of Molecular Sciences 22, no. 22: 12331. https://doi.org/10.3390/ijms222212331