Selectivity Determinants of RHO GTPase Binding to IQGAPs
Abstract
:1. Introduction
2. Results
2.1. IQGAP1/2 Selectively Bind CDC42 and RAC1-Like Members of the RHO Family
2.2. RAC2 Exhibited the Highest Affinity for IQGAP1
2.3. Potential Hotspots for IQGAP Binding Appear Outside the Switch Regions
2.4. PAK1, p50GAP, and DOCK2 Compete with IQGAP1 for Binding RAC1
2.5. IQGAP1 Binding Hotspots Significantly Vary among RHO GTPases
3. Discussion
4. Materials and Methods
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mosaddeghzadeh, N.; Nouri, K.; Krumbach, O.H.F.; Amin, E.; Dvorsky, R.; Ahmadian, M.R. Selectivity Determinants of RHO GTPase Binding to IQGAPs. Int. J. Mol. Sci. 2021, 22, 12596. https://doi.org/10.3390/ijms222212596
Mosaddeghzadeh N, Nouri K, Krumbach OHF, Amin E, Dvorsky R, Ahmadian MR. Selectivity Determinants of RHO GTPase Binding to IQGAPs. International Journal of Molecular Sciences. 2021; 22(22):12596. https://doi.org/10.3390/ijms222212596
Chicago/Turabian StyleMosaddeghzadeh, Niloufar, Kazem Nouri, Oliver H. F. Krumbach, Ehsan Amin, Radovan Dvorsky, and Mohammad R. Ahmadian. 2021. "Selectivity Determinants of RHO GTPase Binding to IQGAPs" International Journal of Molecular Sciences 22, no. 22: 12596. https://doi.org/10.3390/ijms222212596