Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development
Abstract
:1. Introduction
2. Results
2.1. The NlTOR Gene of BPH Is a Target of Rice Osa-miR162a
2.2. Injected Osa-miR162a Mimics Reduce the Fecundity of BPH
2.3. Osa-miR162a-m1 Exhibits Minimal Perturbation on Rice Endogenous Gene Expression
2.4. Rice Development Is Not Defected upon Osa-miR162a-m1 Overexpression
2.5. BPH Adults Fed with miR162a-m1-OE Plants Display Impaired Fecundity
3. Discussion
4. Materials and Methods
4.1. Plant and Insect Materials
4.2. Molecular Cloning and Transforming Rice Plants
4.3. Feeding BPH with Osa-miR162a Mimics
4.4. Injecting BPH with Osa-miR162a Mimics
4.5. Bioassay of the Fecundity
4.6. RT-qPCR or Stem-Loop RT-qPCR
4.7. Feeding BPH with miR162a-OE or miR162a-m1-OE Plants
4.8. BPH Host Choice Test
4.9. Bioinformatic Prediction of miRNA Target Genes
4.10. Statistical Analysis
5. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shen, W.; Cao, S.; Liu, J.; Zhang, W.; Chen, J.; Li, J.-F. Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development. Int. J. Mol. Sci. 2021, 22, 12652. https://doi.org/10.3390/ijms222312652
Shen W, Cao S, Liu J, Zhang W, Chen J, Li J-F. Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development. International Journal of Molecular Sciences. 2021; 22(23):12652. https://doi.org/10.3390/ijms222312652
Chicago/Turabian StyleShen, Wenzhong, Shanni Cao, Jinhui Liu, Wenqing Zhang, Jie Chen, and Jian-Feng Li. 2021. "Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development" International Journal of Molecular Sciences 22, no. 23: 12652. https://doi.org/10.3390/ijms222312652