Targeting S100A9 Reduces Neutrophil Recruitment, Inflammation and Lung Damage in Abdominal Sepsis
Abstract
:1. Introduction
2. Results
2.1. Septic Levels of S100A9
2.2. Neutrophil Recruitment and Mac-1 Expression
2.3. CXC Chemokines and IL-6 in the Lung
2.4. Lung Edema and Tissue Injury
2.5. Systemic Inflammation
2.6. Late Treatment with ABR-238901
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Induction of CLP and Experimental Design
4.3. Myeloperoxidase Activity
4.4. Enzyme-Linked Immunosorbent Assay
4.5. Histology
4.6. Bronchoalveolar Lavage Fluid (BALF) and Lung Edema
4.7. Systemic Leukocytes Differential Counts
4.8. Flow Cytometry
4.9. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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PMNL | MNL | Total Leukocytes | |
---|---|---|---|
Sham | 1.2 ± 0.2 | 5.3 ± 1.9 | 6.5 ± 1.8 |
Vehicle + CLP | 0.6 ± 0.3 # | 2.0 ± 0.8 # | 2.6 ± 1.1 # |
ABR + CLP | 1.3 ± 0.5 * | 3.2 ± 0.7 | 4.6 ± 0.9 * |
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Ding, Z.; Du, F.; Averitt V, R.G.; Jakobsson, G.; Rönnow, C.-F.; Rahman, M.; Schiopu, A.; Thorlacius, H. Targeting S100A9 Reduces Neutrophil Recruitment, Inflammation and Lung Damage in Abdominal Sepsis. Int. J. Mol. Sci. 2021, 22, 12923. https://doi.org/10.3390/ijms222312923
Ding Z, Du F, Averitt V RG, Jakobsson G, Rönnow C-F, Rahman M, Schiopu A, Thorlacius H. Targeting S100A9 Reduces Neutrophil Recruitment, Inflammation and Lung Damage in Abdominal Sepsis. International Journal of Molecular Sciences. 2021; 22(23):12923. https://doi.org/10.3390/ijms222312923
Chicago/Turabian StyleDing, Zhiyi, Feifei Du, Richard Garland Averitt V, Gabriel Jakobsson, Carl-Fredrik Rönnow, Milladur Rahman, Alexandru Schiopu, and Henrik Thorlacius. 2021. "Targeting S100A9 Reduces Neutrophil Recruitment, Inflammation and Lung Damage in Abdominal Sepsis" International Journal of Molecular Sciences 22, no. 23: 12923. https://doi.org/10.3390/ijms222312923