Viral Transduction of Human Rod Opsin or Channelrhodopsin Variants to Mouse ON Bipolar Cells Does Not Impact Retinal Anatomy or Cause Measurable Death in the Targeted Cells
Abstract
:1. Introduction
2. Results
2.1. Expression of Human Rod Opsin in ON Bipolar Cells Using the Grm6cre Mouse
2.2. Gross Retinal Anatomy Is Not Impaired by Viral Delivery of Rod Opsin to ON Bipolar Cells
2.3. ON Bipolar Cell Survival Is Not Impacted by Viral Delivery of Rod Opsin
2.4. Expression of Channelrhodopsin Variants Does Not Impact Gross Retinal Anatomy or Bipolar Cell Number
3. Discussion
4. Methods
4.1. Mice
4.2. Immunohistochemistry
4.3. PCR
4.4. Optical Coherence Tomography
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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ARP Fwd | CGACCTGGAAGTCCAACTAC |
ARP Rev | ATCTGCTGCATCTGCTTG |
GAPDH Fwd | TGCACCACCAACTGCTTAG |
GAPDH Rev | GATGCAGGGATGATGTTC |
PSMB2 Fwd | AAATGCGGAATGGATATGAATTG |
PSMB2 Rev | GAAGACAGTCAGCCAGGTT |
mCherry Fwd | GATAACATGGCCATCATCAAGGA |
mCherry Rev | CGTGGCCGTTCACGGAG |
PKCa/PRKCA Fwd | CGGAAGCCCTACCTTCTGTG |
PKCa/PRKCA Rev | TCTCGTACCGTGACGTGGAG |
hRHO Fwd | CCTGCTCAACCTAGCCGTG |
hRHO Rev | AGGGCAATTTCACCGCCCA |
IBA1/AIF1 Fwd | CAGACTGCCAGCCTAAGACA |
IBA1/AIF1 Rev | AGGAATTGCTTGTTGATCCC |
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Wright, P.; Rodgers, J.; Wynne, J.; Bishop, P.N.; Lucas, R.J.; Milosavljevic, N. Viral Transduction of Human Rod Opsin or Channelrhodopsin Variants to Mouse ON Bipolar Cells Does Not Impact Retinal Anatomy or Cause Measurable Death in the Targeted Cells. Int. J. Mol. Sci. 2021, 22, 13111. https://doi.org/10.3390/ijms222313111
Wright P, Rodgers J, Wynne J, Bishop PN, Lucas RJ, Milosavljevic N. Viral Transduction of Human Rod Opsin or Channelrhodopsin Variants to Mouse ON Bipolar Cells Does Not Impact Retinal Anatomy or Cause Measurable Death in the Targeted Cells. International Journal of Molecular Sciences. 2021; 22(23):13111. https://doi.org/10.3390/ijms222313111
Chicago/Turabian StyleWright, Phillip, Jessica Rodgers, Jonathan Wynne, Paul N. Bishop, Robert J. Lucas, and Nina Milosavljevic. 2021. "Viral Transduction of Human Rod Opsin or Channelrhodopsin Variants to Mouse ON Bipolar Cells Does Not Impact Retinal Anatomy or Cause Measurable Death in the Targeted Cells" International Journal of Molecular Sciences 22, no. 23: 13111. https://doi.org/10.3390/ijms222313111