Cell Penetrating Peptide-Based Self-Assembly for PD-L1 Targeted Tumor Regression
Abstract
:1. Introduction
2. Results
2.1. Characterization of Peptide and Peptide-RNA/DOX
2.2. Internalization of Peptide-RNA/DOX and Its Mechanism
2.3. Anti-Tumor Effects In Vitro
2.4. Anti-Tumor Effects In Vivo
3. Discussion
4. Materials and Methods
4.1. Reagent
4.2. Synthesis of Peptide and Preparation of Nano Vehicles
4.3. Gel Retardation Assay and Drug Loading Efficiency
4.4. Characterization
4.5. Hemolysis Test
4.6. Cell Culture and MTT Assays
4.7. Internalization of Nano Vehicles and Its Mechanism
4.8. Cell Apoptosis
4.9. Quantitative Real-Time-PCR and Western Blot Assays
4.10. In Vivo Administrations of Drug
4.11. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
PD-L1 | Programmed cell death 1 ligand 1 |
FF | Phe-Phe |
PEI | polyethyleneimine |
PLL | poly-(l-lysine) |
PCL | Polycaprolactone |
IAP | inhibitor of apoptosis protein |
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Guo, F.; Ke, J.; Fu, Z.; Han, W.; Wang, L. Cell Penetrating Peptide-Based Self-Assembly for PD-L1 Targeted Tumor Regression. Int. J. Mol. Sci. 2021, 22, 13314. https://doi.org/10.3390/ijms222413314
Guo F, Ke J, Fu Z, Han W, Wang L. Cell Penetrating Peptide-Based Self-Assembly for PD-L1 Targeted Tumor Regression. International Journal of Molecular Sciences. 2021; 22(24):13314. https://doi.org/10.3390/ijms222413314
Chicago/Turabian StyleGuo, Feng, Junfeng Ke, Zhengdong Fu, Wenzhao Han, and Liping Wang. 2021. "Cell Penetrating Peptide-Based Self-Assembly for PD-L1 Targeted Tumor Regression" International Journal of Molecular Sciences 22, no. 24: 13314. https://doi.org/10.3390/ijms222413314