Jmjd3 Mediates Neuropathic Pain by Inducing Macrophage Infiltration and Activation in Lumbar Spinal Stenosis Animal Model
Abstract
:1. Introduction
2. Results
2.1. Jmjd3 Expression and Activity Is Increased after Cauda Equine Compression in an LSS Model
2.2. Jmjd3 Regulates Blood-Nerve Barrier Permeability after Cauda Equine Compression in an LSS Model
2.3. Jmjd3 Inhibitor Reduces Macrophage Infiltration and Inflammation in Injured Cauda Equina of LSS Rats
2.4. Jmjd3 Regulates the Expression and Activity of MMP-2/9 in Cauda Equina after Compression Injury
2.5. Jmjd3 Also Regulates Macrophage Activation
2.6. The Inhibition of Jmjd3 Activity Alleviates LSS-Induced Mechanical Allodynia
3. Discussion
4. Materials and Methods
4.1. Animals and Ethics Statement
4.2. Cauda Equina Compression Injury
4.3. Pain Behavioral Tests
4.4. Drug Administration
4.5. Tissue Preparation
4.6. Immunohistochemistry and Immunocytochemistry
4.7. Western Blot
4.8. Gelatin Zymography
4.9. RNA Isolation and RT-PCR
4.10. Endothelial Cell Culture and OGD/Reperfusion
4.11. Measurement of Transendothelial Electrical Resistance (TEER)
4.12. RAW 264.7 Macrophages Culture
4.13. Determination of NO and PGE2 Production
4.14. Measurement of Blood-Nerve Barrier Permeability
4.15. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lee, J.; Choi, H.; Park, C.; Jeon, S.; Yune, T. Jmjd3 Mediates Neuropathic Pain by Inducing Macrophage Infiltration and Activation in Lumbar Spinal Stenosis Animal Model. Int. J. Mol. Sci. 2021, 22, 13426. https://doi.org/10.3390/ijms222413426
Lee J, Choi H, Park C, Jeon S, Yune T. Jmjd3 Mediates Neuropathic Pain by Inducing Macrophage Infiltration and Activation in Lumbar Spinal Stenosis Animal Model. International Journal of Molecular Sciences. 2021; 22(24):13426. https://doi.org/10.3390/ijms222413426
Chicago/Turabian StyleLee, Jeeyoun, Haeyoung Choi, Chansol Park, Sangryong Jeon, and Taeyoung Yune. 2021. "Jmjd3 Mediates Neuropathic Pain by Inducing Macrophage Infiltration and Activation in Lumbar Spinal Stenosis Animal Model" International Journal of Molecular Sciences 22, no. 24: 13426. https://doi.org/10.3390/ijms222413426