Genome-Wide Identification, Characterization and Expression Analysis of the CIPK Gene Family in Potato (Solanum tuberosum L.) and the Role of StCIPK10 in Response to Drought and Osmotic Stress
Abstract
:1. Introduction
2. Results
2.1. Identification, Gene Duplication and the Phylogenetic Tree of StCIPK Family Genes
2.2. Analysis of Gene Structure and Cis-Acting Elements
2.3. Expression Analysis of StCIPK Genes in Different Tissues
2.4. Expression Analysis of StCIPK Genes under Different Abiotic Stresses and ABA treatment
2.5. StCIPK10 Positively Modulates Responses to Drought and Osmotic Stress in Potato
2.6. Overexpression of StCIPK10 Increases ABA Sensitivity and Stomatal Closure in Transgenic Potato
2.7. StCIPK10 Regulates Expression of Stress-Induced Genes in Transgenic Potato
2.8. Interaction between StCIPK10 and StCBL Proteins
3. Discussion
3.1. StCIPK Gene Expansion and Evolution in Potato
3.2. StCIPK10 is a Positive Regulator of Potato Tolerance to Drought and Osmotic Stress of Potato
3.3. StCIPK10 Is a Positive Regulator of Potato Tolerance to Drought and Osmotic Stress
3.4. StCIPK10 Is a Positive Regulator of ABA-Dependent Responses
3.5. Interactive Mechanism of StCIPK10 with Upstream Regulators
4. Materials and Methods
4.1. Identification of StCIPK Genes
4.2. Chromosomal Localization, Phylogenetic and Gene Duplication Analysis
4.3. Cis-Acting Element and Gene Structure Analysis
4.4. Plant Materials and Treatments
4.5. RNA Isolation and Gene Expression Analysis
4.6. Subcelluar Localization Analysis of StCIPK10
4.7. Protein Interaction Assay
4.8. Production of Transgenic Plants
4.9. Assays for Drought and Osmotic Stress Tolerance
4.10. Assays for Stomatal Movement and Phenotype under ABA Treatment
4.11. Validation of amiRNA Target Genes using RLM 5′-RACE
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene ID | Chr. 1 | Genomic Location (bp) | CDS Length (bp) 2 | No. of Exons | Protein Length (aa) 3 | MW (kDa) 4 | pI 5 |
---|---|---|---|---|---|---|---|---|
StCIPK01 | Soltu.DM.01G005660.2 | 1 | 5,154,274–5,159,709 | 1317 | 14 | 438 | 50.23 | 6.72 |
StCIPK02 | Soltu.DM.02G002430.1 | 2 | 11,576,701–11,584,119 | 1368 | 14 | 455 | 51.10 | 8.92 |
StCIPK03 | Soltu.DM.02G015750.1 | 2 | 31,880,731–31,885,458 | 1437 | 1 | 478 | 53.76 | 8.60 |
StCIPK04 | Soltu.DM.02G015770.1 | 2 | 31,899,161–31,901,152 | 1377 | 2 | 458 | 52.14 | 9.05 |
StCIPK05 | Soltu.DM.03G000420.1 | 3 | 383,299–385,208 | 1302 | 1 | 433 | 48.63 | 9.26 |
StCIPK06 | Soltu.DM.03G001400.1 | 3 | 1,308,274–3,10,351 | 1113 | 2 | 370 | 42.44 | 8.59 |
StCIPK07 | Soltu.DM.04G031510.2 | 4 | 65,585,586–65,591,402 | 1344 | 14 | 447 | 50.63 | 6.38 |
StCIPK08 | Soltu.DM.05G019250.1 | 5 | 43,311,668–43,315,613 | 1485 | 12 | 494 | 56.43 | 7.64 |
StCIPK09 | Soltu.DM.05G022320.1 | 5 | 47,506,266–47,510,051 | 1338 | 1 | 445 | 50.20 | 9.07 |
StCIPK10 | Soltu.DM.05G023210.1 | 5 | 48,492,654–48,497,748 | 1371 | 12 | 456 | 51.44 | 6.45 |
StCIPK11 | Soltu.DM.06G002800.1 | 6 | 2,846,349–2,848,922 | 1341 | 2 | 446 | 50.96 | 8.47 |
StCIPK12 | Soltu.DM.06G002810.1 | 6 | 2,851,745–2,855,743 | 1413 | 1 | 470 | 53.82 | 8.27 |
StCIPK13 | Soltu.DM.06G010870.1 | 6 | 31,710,868–31,712,193 | 1326 | 1 | 441 | 49.74 | 9.10 |
StCIPK14 | Soltu.DM.06G010880.1 | 6 | 31,745,058–31,746,792 | 1332 | 1 | 443 | 50.07 | 8.90 |
StCIPK15 | Soltu.DM.06G024260.1 | 6 | 50,088,724–50,090,478 | 1353 | 1 | 450 | 51.02 | 8.92 |
StCIPK16 | Soltu.DM.06G032750.1 | 6 | 57,566,917–57,568,428 | 1260 | 2 | 419 | 48.00 | 8.27 |
StCIPK17 | Soltu.DM.07G000510.1 | 7 | 636,765–638,572 | 1275 | 1 | 424 | 47.66 | 9.08 |
StCIPK18 | Soltu.DM.09G010080.1 | 7 | 18,714,464–18,716,381 | 1413 | 1 | 470 | 53.04 | 8.59 |
StCIPK19 | Soltu.DM.08G015880.1 | 8 | 40,832,281–40,834,116 | 1371 | 1 | 456 | 52.35 | 8.63 |
StCIPK20 | Soltu.DM.09G010290.1 | 9 | 14,851,369–14,853,146 | 1353 | 1 | 450 | 50.95 | 8.54 |
StCIPK21 | Soltu.DM.09G025570.1 | 9 | 55,141,943–55,143,603 | 1317 | 2 | 437 | 49.44 | 8.90 |
StCIPK22 | Soltu.DM.10G022340.1 | 10 | 53,624,978–53,626,957 | 1311 | 1 | 436 | 49.23 | 9.36 |
StCIPK23 | Soltu.DM.11G018660.1 | 11 | 35,353,412–35,361,571 | 1398 | 13 | 465 | 53.07 | 7.24 |
StCIPK24 | Soltu.DM.12G027440.1 | 12 | 2,229,648–2,31,821 | 1299 | 1 | 432 | 48.60 | 9.00 |
StCIPK25 | Soltu.DM.12G026670.4 | 12 | 2,830,602–2,838,182 | 1341 | 14 | 446 | 50.63 | 8.87 |
StCIPK26 | Soltu.DM.12G018730.1 | 12 | 18,210,942–18,212,405 | 1464 | 1 | 487 | 54.77 | 9.18 |
StCIPK27 | Soltu.DM.12G002020.1 | 12 | 59,751,790–59,759,517 | 1596 | 11 | 531 | 60.01 | 5.97 |
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Ma, R.; Liu, W.; Li, S.; Zhu, X.; Yang, J.; Zhang, N.; Si, H. Genome-Wide Identification, Characterization and Expression Analysis of the CIPK Gene Family in Potato (Solanum tuberosum L.) and the Role of StCIPK10 in Response to Drought and Osmotic Stress. Int. J. Mol. Sci. 2021, 22, 13535. https://doi.org/10.3390/ijms222413535
Ma R, Liu W, Li S, Zhu X, Yang J, Zhang N, Si H. Genome-Wide Identification, Characterization and Expression Analysis of the CIPK Gene Family in Potato (Solanum tuberosum L.) and the Role of StCIPK10 in Response to Drought and Osmotic Stress. International Journal of Molecular Sciences. 2021; 22(24):13535. https://doi.org/10.3390/ijms222413535
Chicago/Turabian StyleMa, Rui, Weigang Liu, Shigui Li, Xi Zhu, Jiangwei Yang, Ning Zhang, and Huaijun Si. 2021. "Genome-Wide Identification, Characterization and Expression Analysis of the CIPK Gene Family in Potato (Solanum tuberosum L.) and the Role of StCIPK10 in Response to Drought and Osmotic Stress" International Journal of Molecular Sciences 22, no. 24: 13535. https://doi.org/10.3390/ijms222413535