Partial Inhibition of Calcineurin Activity by Rcn2 as a Potential Remedy for Vps13 Deficiency
Abstract
:1. Introduction
2. Results
2.1. The RCN2 Gene is a Multicopy Suppressor of vps13Δ SDS Hypersensitivity
2.2. Binding of Rcn2 to Slt2 Kinase is Not Important for Suppression
2.3. The Suppression of vps13Δ by RCN2 Requires Calcineurin
2.4. Both rcn2-m1 and rcn2-m3 Mutations Abolish the Binding of Rcn2 to Catalytic Subunit of Calcineurin, Cmp2
2.5. Partial Inhibition of Calcineurin Activity is Necessary for Suppression
2.6. Rcn2 Binds to the Cmp2 Fragment Corresponding to aa 382–455
3. Discussion
4. Materials and Methods
4.1. Strains, Media, and Growth Conditions
4.2. Plasmids, Mutagenesis, and Screen for Multicopy Suppressors of vps13 Mutations
4.3. β-Galactosidase Activity Assay
4.4. Protein Extracts and Western Blot Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wardaszka, P.; Soczewka, P.; Sienko, M.; Zoladek, T.; Kaminska, J. Partial Inhibition of Calcineurin Activity by Rcn2 as a Potential Remedy for Vps13 Deficiency. Int. J. Mol. Sci. 2021, 22, 1193. https://doi.org/10.3390/ijms22031193
Wardaszka P, Soczewka P, Sienko M, Zoladek T, Kaminska J. Partial Inhibition of Calcineurin Activity by Rcn2 as a Potential Remedy for Vps13 Deficiency. International Journal of Molecular Sciences. 2021; 22(3):1193. https://doi.org/10.3390/ijms22031193
Chicago/Turabian StyleWardaszka, Patrycja, Piotr Soczewka, Marzena Sienko, Teresa Zoladek, and Joanna Kaminska. 2021. "Partial Inhibition of Calcineurin Activity by Rcn2 as a Potential Remedy for Vps13 Deficiency" International Journal of Molecular Sciences 22, no. 3: 1193. https://doi.org/10.3390/ijms22031193