Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Generation of Transgenic Rice Cell Lines Harboring αAmy3p-SP-Oct4 Gene
2.2. Recombinant Human Oct4 Proteins Were Produced by Transgenic Rice Suspension Cell Lines
2.3. The rhOct4 Protein Is Unstable in Sugar-Free Culture Medium
2.4. The Highest Production of Oct4 Protein Was 0.41% of Total Soluble Proteins
2.5. Rice Cells Produce Biologically Active rhOct4
3. Materials and Methods
3.1. Plant Materials and Growth Conditions
3.2. Plasmid Construction
3.3. Rice Transformation
3.4. PCR-Base Genotype Analysis
3.5. Quantitative RT-PCR
3.6. Western Blot Analysis
3.7. Oct4 Transcription Factor Binding Assay
3.8. In-Gel Protease Activity Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
Oct4 | Octamer-binding Transcription Factor 4 |
iPSCs | induced pluripotent stem cells |
qRT-PCR | quantitative real-time PCR |
MS medium | Murashige and Skoog medium |
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Huang, L.-F.; Sinaga, D.S.; Tan, C.-C.; Hsieh, S.-J.M.; Huang, C.-H. Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells. Int. J. Mol. Sci. 2021, 22, 1409. https://doi.org/10.3390/ijms22031409
Huang L-F, Sinaga DS, Tan C-C, Hsieh S-JM, Huang C-H. Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells. International Journal of Molecular Sciences. 2021; 22(3):1409. https://doi.org/10.3390/ijms22031409
Chicago/Turabian StyleHuang, Li-Fen, Desyanti Saulina Sinaga, Chia-Chun Tan, Shu-Ju Micky Hsieh, and Chi-Hung Huang. 2021. "Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells" International Journal of Molecular Sciences 22, no. 3: 1409. https://doi.org/10.3390/ijms22031409