MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake
Abstract
:1. Introduction
2. Results
2.1. An Increase in MST3 Levels in Mouse Kidneys with Increasing K+ Intake
2.2. Reduction of Diuresis, Kaliuresis, and Natriuresis in MST3−/− Mice Fed 2% KCl Diets
2.3. WNK4 and WNK4-Regulated Channels in 2% KCl Diet-Fed Mice
3. Discussion
4. Methods
4.1. Animals
4.2. Immunohistochemistry
4.3. Immunoblotting
4.4. Measurement of Blood Pressure, Serum Na+, and Urinary Concentrations of Na+ and K+ and Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diet | Control Diet (0.43% Na, 1.1% K) (n = 5) | High Salt (8% Na, 1.1% K) (n = 5) |
---|---|---|
Weight, g | 19.55 ± 2.12 | 21.94 ± 1.52 |
Water intake, mL | 4.96 ± 0.52 | 8.62 ± 1.01 |
Food intake, g | 3.17 ± 0.43 | 8.82 ± 1.89 |
Value | |||
---|---|---|---|
16 Days | MST3+/+ (WT) | MST3 −/− | |
Plasma [Na+] (mmol/L) | control diet | 152.33 ± 0.52 | 153.4 ± 0.89 * |
2% KCl diets | 152.5 ± 0.84 | 154.67 ± 1.53 * | |
Blood pressure (mmHg) | control diet | 117 ± 9 | 130 ± 13 * |
2% KCl diets | 118 ± 10 | 131 ± 9 * |
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Chan, C.-H.; Wu, S.-N.; Bao, B.-Y.; Li, H.-W.; Lu, T.-L. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. Int. J. Mol. Sci. 2021, 22, 999. https://doi.org/10.3390/ijms22030999
Chan C-H, Wu S-N, Bao B-Y, Li H-W, Lu T-L. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. International Journal of Molecular Sciences. 2021; 22(3):999. https://doi.org/10.3390/ijms22030999
Chicago/Turabian StyleChan, Chee-Hong, Sheng-Nan Wu, Bo-Ying Bao, Houng-Wei Li, and Te-Ling Lu. 2021. "MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake" International Journal of Molecular Sciences 22, no. 3: 999. https://doi.org/10.3390/ijms22030999