Gelatin Methacryloyl–Riboflavin (GelMA–RF) Hydrogels for Bone Regeneration
Abstract
:1. Introduction
2. Results
2.1. Physical and Mechanical Properties of GelMA–RF Hydrogels
2.2. Cell Viability Following VW and UV Light Photopolymerization
2.3. Osteoblastic Differentiation of KUSA-A1 Cells in a 2D Environment
2.4. Expression of Osteogenesis-Related Genes in Osteogenic Induced KUSA-A1 Cells Encapsulated in GelMA
3. Discussion
4. Materials and Methods
4.1. GelMA Hydrogel Preparation
4.2. Determination of the Polymerization Conditions of GelMA Hydrogels
4.3. Mechanical Stability
4.4. Cell Culture and Osteo-Induction in a Two-Dimensional (2D) Environment
4.5. Cell Encapsulation and Cell Viability in a 3D Environment
4.6. Cell Encapsulation and Osteo-Induction in a 3D Environment
4.7. qPCR Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
GelMA | Gelatin methacryloyl |
UV | Ultraviolet |
VW | Visible wavelength |
RF | Riboflavin |
MA | Methacrylic anhydride |
IR | Irgacure2959 |
Runx2 | Runt-Related Transcription Factor 2 |
Osx | Osterix |
Bsp | Bone Sialoprotein |
Ocn | Osteocalcin |
Appendix A
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Goto, R.; Nishida, E.; Kobayashi, S.; Aino, M.; Ohno, T.; Iwamura, Y.; Kikuchi, T.; Hayashi, J.-i.; Yamamoto, G.; Asakura, M.; et al. Gelatin Methacryloyl–Riboflavin (GelMA–RF) Hydrogels for Bone Regeneration. Int. J. Mol. Sci. 2021, 22, 1635. https://doi.org/10.3390/ijms22041635
Goto R, Nishida E, Kobayashi S, Aino M, Ohno T, Iwamura Y, Kikuchi T, Hayashi J-i, Yamamoto G, Asakura M, et al. Gelatin Methacryloyl–Riboflavin (GelMA–RF) Hydrogels for Bone Regeneration. International Journal of Molecular Sciences. 2021; 22(4):1635. https://doi.org/10.3390/ijms22041635
Chicago/Turabian StyleGoto, Ryoma, Eisaku Nishida, Shuichiro Kobayashi, Makoto Aino, Tasuku Ohno, Yuki Iwamura, Takeshi Kikuchi, Jun-ichiro Hayashi, Genta Yamamoto, Masaki Asakura, and et al. 2021. "Gelatin Methacryloyl–Riboflavin (GelMA–RF) Hydrogels for Bone Regeneration" International Journal of Molecular Sciences 22, no. 4: 1635. https://doi.org/10.3390/ijms22041635