Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts
Abstract
:1. Introduction
2. Results
2.1. Clinical Characterization of Patients and Tumors Used for CAFs Preparation
2.2. Lineage Specificity of Patient-Derived CAFs and Preparation of Conditioned Media
2.3. Patient-Derived CAFs Affect the Colony-Forming Capability of Cancer Cells
2.4. The Colony-Forming Capability of FaDu Cells after CAF Coculture Is Related to Cancer-Associated Genes
2.5. Cisplatin Response in FaDu Cells Is CAF-Specific
2.6. Platin Amount Accumulated to and Released from CAFs Relates to Aggressiveness
3. Discussion
4. Materials and Methods
4.1. Model Cell Lines
4.2. Tumor Samples Collection
4.3. Patient-Derived Cell Cultures
4.4. Colony-Forming Assay
4.5. Gene Expression Analysis
4.6. RNA Isolation and Reverse Transcription
4.7. Quantitative Real-Time Polymerase Chain Reaction
4.8. Cisplatin Concentration in CAF-Derived Media
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patient | Gender | Age at Diagnosis | Tumor Location | pT | pN | cM | G | p16 | Stage | Surgery + Adjuvant Radiotherapy | Adjuvant Chemotherapy | Recurrence |
---|---|---|---|---|---|---|---|---|---|---|---|---|
M5.1 | F | 59 | larynx | 4a | 2b | 0 | 3 | 0 | IVA | 1 | 0 | 0 |
M6.1 | M | 81 | oropharynx | 2 | 0 | 0 | 2 | 0 | I | 1 | 0 | 0 |
M7.1 | M | 60 | hypopharynx | 2 | 2b | 0 | 2 | 1 | IVA | 1 | 0 | 1 |
M9.1 | M | 54 | oropharynx | 2 | 1 | 0 | 2 | 1 | I | 1 | 1 | 1 |
M11.1 | M | 63 | oropharynx | 2 | 2 | 0 | 3 | 1 | II | 1 | 1 | 0 |
M12.1 | M | 49 | larynx | 4a | 1 | 0 | 3 | 0 | IVA | 1 | 0 | 0 |
M14.1 | M | 62 | larynx | 4a | 0 | 0 | 2 | 0 | IVA | 1 | 0 | 0 |
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Peltanova, B.; Liskova, M.; Gumulec, J.; Raudenska, M.; Polanska, H.H.; Vaculovic, T.; Kalfert, D.; Grega, M.; Plzak, J.; Betka, J.; et al. Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts. Int. J. Mol. Sci. 2021, 22, 1912. https://doi.org/10.3390/ijms22041912
Peltanova B, Liskova M, Gumulec J, Raudenska M, Polanska HH, Vaculovic T, Kalfert D, Grega M, Plzak J, Betka J, et al. Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts. International Journal of Molecular Sciences. 2021; 22(4):1912. https://doi.org/10.3390/ijms22041912
Chicago/Turabian StylePeltanova, Barbora, Marketa Liskova, Jaromir Gumulec, Martina Raudenska, Hana Holcova Polanska, Tomas Vaculovic, David Kalfert, Marek Grega, Jan Plzak, Jan Betka, and et al. 2021. "Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts" International Journal of Molecular Sciences 22, no. 4: 1912. https://doi.org/10.3390/ijms22041912