A Role for Mycobacterium tuberculosis Sigma Factor C in Copper Nutritional Immunity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Absence of Significant Growth or Global Gene Expression Differences after Deletion of sigC from M. tuberculosis Strain Erdman Cultured in 7H9 Medium
2.2. Artificial sigC Induction Points to a Role for SigC in Metal Transport
2.3. Identification of an In Vitro Phenotype for ΔsigC
2.4. Differential Gene Expression between Erdman and ΔsigC in SMT Supports SigC Function in Copper Uptake
2.5. Copper Supplementation Eliminates Most Transcriptional Differences between ΔsigC and Erdman
2.6. Complete Reversal of the ΔsigC Growth Defect by 25 µM Copper Sulfate Addition
2.7. Attenuation of M. tuberculosis Erdman ΔsigC Virulence in SCID Mice
2.8. Future Directions
3. Materials and Methods
3.1. Bacterial Strains and Culture Conditions
3.2. RNA Isolation
3.3. Microarray Analyses
3.4. Quantitative RT-PCR Assays
3.5. Animal Infection Studies
3.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Grosse-Siestrup, B.T.; Gupta, T.; Helms, S.; Tucker, S.L.; Voskuil, M.I.; Quinn, F.D.; Karls, R.K. A Role for Mycobacterium tuberculosis Sigma Factor C in Copper Nutritional Immunity. Int. J. Mol. Sci. 2021, 22, 2118. https://doi.org/10.3390/ijms22042118
Grosse-Siestrup BT, Gupta T, Helms S, Tucker SL, Voskuil MI, Quinn FD, Karls RK. A Role for Mycobacterium tuberculosis Sigma Factor C in Copper Nutritional Immunity. International Journal of Molecular Sciences. 2021; 22(4):2118. https://doi.org/10.3390/ijms22042118
Chicago/Turabian StyleGrosse-Siestrup, Benjamin T., Tuhina Gupta, Shelly Helms, Samantha L. Tucker, Martin I. Voskuil, Frederick D. Quinn, and Russell K. Karls. 2021. "A Role for Mycobacterium tuberculosis Sigma Factor C in Copper Nutritional Immunity" International Journal of Molecular Sciences 22, no. 4: 2118. https://doi.org/10.3390/ijms22042118