Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet
Abstract
:1. Introduction
2. Results
2.1. Body Weight, Food Intake, Beverage Intake and Osthol Dosing
2.2. Renal Effects of an HF/HS Diet
2.3. Systemic Markers of Metabolic Alterations and Blood Pressure
2.4. Fructokinase Expression and Activity Assessment and Markers of Fructokinase Activation
2.5. Assessment of Renal Lipid Accumulation
2.6. Oxidative Stress
3. Discussion
4. Materials and Methods
4.1. Ethical Approval
4.2. Experimental Protocol
4.3. Body Weight and Feeding
4.4. Systolic Blood Pressure (SBP)
4.5. Kidney Function
4.6. Fructokinase Activity
4.7. Plasma Parameters
4.8. Intrarenal Fructose, Uric Acid, and Triglycerides
4.9. Western Blot Analysis
4.10. Oxidative Stress Markers
4.11. Reagents
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diet | Composition (% from Total Weight) | Nutritional Value (% of Total Kilocalories) |
---|---|---|
Regular | Commercially available: Lab Diet’s rodent diet 5010 | Protein 28.66 Fat 13.11 Carbohydrate 58.22 |
HF/HS | 1% Cholesterol 0.5% Cholic acid 5% Butter 30% Powdered sugar 10% Casein 2% NaCl 51.5% Regular diet | Protein 23.10 Fat 20.93 Carbohydrate 56.46 |
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García-Arroyo, F.E.; Gonzaga-Sánchez, G.; Tapia, E.; Muñoz-Jiménez, I.; Manterola-Romero, L.; Osorio-Alonso, H.; Arellano-Buendía, A.S.; Pedraza-Chaverri, J.; Roncal-Jiménez, C.A.; Lanaspa, M.A.; et al. Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet. Int. J. Mol. Sci. 2021, 22, 2431. https://doi.org/10.3390/ijms22052431
García-Arroyo FE, Gonzaga-Sánchez G, Tapia E, Muñoz-Jiménez I, Manterola-Romero L, Osorio-Alonso H, Arellano-Buendía AS, Pedraza-Chaverri J, Roncal-Jiménez CA, Lanaspa MA, et al. Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet. International Journal of Molecular Sciences. 2021; 22(5):2431. https://doi.org/10.3390/ijms22052431
Chicago/Turabian StyleGarcía-Arroyo, Fernando E., Guillermo Gonzaga-Sánchez, Edilia Tapia, Itzel Muñoz-Jiménez, Lino Manterola-Romero, Horacio Osorio-Alonso, Abraham S. Arellano-Buendía, José Pedraza-Chaverri, Carlos A. Roncal-Jiménez, Miguel A. Lanaspa, and et al. 2021. "Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet" International Journal of Molecular Sciences 22, no. 5: 2431. https://doi.org/10.3390/ijms22052431