Effects of a Catechol-Functionalized Hyaluronic Acid Patch Combined with Human Adipose-Derived Stem Cells in Diabetic Wound Healing
Abstract
:1. Introduction
2. Results
2.1. Wound Area Measurement
2.2. PKH26-Labeled ADSC Tracing
2.3. Histopathological Assessment
2.4. Tissue Neovascularization
2.5. Angiogenesis-Related Gene Expression Profile
2.6. VEGF Expression Analysis by Immunofluorescence
2.7. Angiogenesis-Related Protein Expression
3. Discussion
4. Materials and Methods
4.1. HA-CA Synthesis
4.2. ADSC Isolation and Characterization
4.3. Diabetic Wound Animal Model
4.4. Histopathological Assessment
4.5. Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Staining
4.6. Immunohistochemistry
4.7. RNA Isolation and qRT-PCR
4.8. Immunofluorescence Analysis
4.9. Protein Extraction and Western Blotting
4.10. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Sequences |
---|---|
VEGF | F: 5′-GCA CAT AGA GAG AAT GAG CTT CC-3′ R: 5′-CTC CGC TCT GAA CAA GGC T-3′ |
ANG-1 | F: 5′- ATC TTG ATA ACC GCA GCC AC-3′ R: 5′-TGT CGG CAC ATA CCT CTT GT-3′ |
IGF-1 | F: 5′-ATG TAC TGT GCC CCA CTG AAG-3′ R: 5′-GTG TTT CGA TGT TTT GCA GGT-3′ |
FGF-2 | F: 5′-GCT GGC TTC TAA GTG TGT-3′ R: 5′-CCA ACT GGA GTA TTT CCG TGA-3′ |
PI3K | F: 5′-CTC TCC TGT GCT GGC TAC TGT-3′ R: 5′-GCT CTC GGT TGA TTC CAA ACT-3′ |
AKT | F: 5′-ATG AAC GAC GTA GCC ATT GTG-3′ R: 5′-TTG TAG CCA ATA AAG GTG CCA T-3′ |
Spred-1 | F: 5′-GAT GAG CGA GGA GAC GGC GAC-3′ R: 5′-GTC TCT GAG TCT CTC TCC ACG GA-3′ |
ERK1 | F: 5′-GCG TTA CAT GTG GCA GCT TGA-3′ R: 5′-TGG AAC CCC ACC CCA TTT T-3′ |
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Pak, C.S.; Heo, C.Y.; Shin, J.; Moon, S.Y.; Cho, S.-W.; Kang, H.J. Effects of a Catechol-Functionalized Hyaluronic Acid Patch Combined with Human Adipose-Derived Stem Cells in Diabetic Wound Healing. Int. J. Mol. Sci. 2021, 22, 2632. https://doi.org/10.3390/ijms22052632
Pak CS, Heo CY, Shin J, Moon SY, Cho S-W, Kang HJ. Effects of a Catechol-Functionalized Hyaluronic Acid Patch Combined with Human Adipose-Derived Stem Cells in Diabetic Wound Healing. International Journal of Molecular Sciences. 2021; 22(5):2632. https://doi.org/10.3390/ijms22052632
Chicago/Turabian StylePak, Chang Sik, Chan Yeong Heo, Jisoo Shin, Soo Young Moon, Seung-Woo Cho, and Hyo Jin Kang. 2021. "Effects of a Catechol-Functionalized Hyaluronic Acid Patch Combined with Human Adipose-Derived Stem Cells in Diabetic Wound Healing" International Journal of Molecular Sciences 22, no. 5: 2632. https://doi.org/10.3390/ijms22052632