Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana
Abstract
:1. Introduction
2. Results
2.1. Subcellular Localisation of Arabidopsis LPEAT1 and LPEAT2
2.2. NBR1 Transcript Levels and NBR1 Protein Abundance in Rosette Leaves of Wild-Type and LPEAT Transgenic Arabidopsis Plants
2.3. ATG8a Transcript Levels and ATG8 Protein Abundance in Rosette Leaves of Wild-Type and LPEAT Transgenic Arabidopsis Plants
2.4. Relative Amount of Different Forms of ATG8 Proteins in Rosette Leaves of Wild Type Arabidopsis Plants and Plants with Knocked-Out or Overexpressed LPEAT
2.5. Ultrastructure of Cells of Wild Type Plants and Lpeat Mutants of A. thaliana
2.6. Lifespan of Wild-Type Arabidopsis Plants and Plants with Knocked-Out or Overexpressed LPEAT
3. Discussion
3.1. Subcellular Localisation of LPEAT1 and LPEAT2
3.2. Autophagy Markers Levels
3.3. Ultrastructure of Mesophyll Cells
3.4. Life Span of Tested Arabidopsis Lines
3.5. Conclusions
4. Methods
4.1. Plant Materials and Growth Conditions
4.2. Subcellular Localisation of LPEAT1 and LPEAT2 in BY-2 Cells
4.3. Determination of NBR1 and ATG8 Protein Levels in Leaves of A. thaliana
4.4. Measuring ATG8a and NBR1 Expression Levels in Leaves of A. thaliana by q-RT-PCR
4.5. Ultrastructural Analysis of Cells of Leaves of Wild Type Plants and Lpeat Mutants of A. thaliana
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jasieniecka-Gazarkiewicz, K.; Demski, K.; Gidda, S.K.; Klińska, S.; Niedojadło, J.; Lager, I.; Carlsson, A.S.; Minina, E.A.; Mullen, R.T.; Bozhkov, P.V.; et al. Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana. Int. J. Mol. Sci. 2021, 22, 3006. https://doi.org/10.3390/ijms22063006
Jasieniecka-Gazarkiewicz K, Demski K, Gidda SK, Klińska S, Niedojadło J, Lager I, Carlsson AS, Minina EA, Mullen RT, Bozhkov PV, et al. Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana. International Journal of Molecular Sciences. 2021; 22(6):3006. https://doi.org/10.3390/ijms22063006
Chicago/Turabian StyleJasieniecka-Gazarkiewicz, Katarzyna, Kamil Demski, Satinder K. Gidda, Sylwia Klińska, Janusz Niedojadło, Ida Lager, Anders S. Carlsson, Elena A. Minina, Robert T. Mullen, Peter V. Bozhkov, and et al. 2021. "Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana" International Journal of Molecular Sciences 22, no. 6: 3006. https://doi.org/10.3390/ijms22063006