LATS1 Is a Mediator of Melanogenesis in Response to Oxidative Stress and Regulator of Melanoma Growth
Abstract
:1. Introduction
2. Results
2.1. LATS1 Determines Melanin Production and Expression of Melanogenesis Markers in Primary Melanocytes and Melanoma Cells
2.2. LATS1 Regulates the Proliferation of Primary Melanocytes and Determines Tumor Growth Kinetics
2.3. LATS1 Knockdown Enhances Reactive Oxygen Species Production in Primary Melanocytes and Melanoma Cells
2.4. LATS1 Regulates Oxidative Stress-Responsive and Cellular Stress-Related Genes Expression in Melanocytes but Not in Melanoma Cells
2.5. LATS1 Regulates the Expression of Hypoxia-Related Genes
2.6. LATS1 Silencing Drives Minor Changes in YAP1/TAZ Expression in Primary Melanocytes but Not in MeWo Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Lentivirus Production and Transduction
4.3. RNA Sequencing
4.4. Protein Extraction and Western Blot
4.5. Oxidative Stress Detection
4.6. Mice
4.7. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kazimierczak, U.; Dondajewska, E.; Zajaczkowska, M.; Karwacka, M.; Kolenda, T.; Mackiewicz, A. LATS1 Is a Mediator of Melanogenesis in Response to Oxidative Stress and Regulator of Melanoma Growth. Int. J. Mol. Sci. 2021, 22, 3108. https://doi.org/10.3390/ijms22063108
Kazimierczak U, Dondajewska E, Zajaczkowska M, Karwacka M, Kolenda T, Mackiewicz A. LATS1 Is a Mediator of Melanogenesis in Response to Oxidative Stress and Regulator of Melanoma Growth. International Journal of Molecular Sciences. 2021; 22(6):3108. https://doi.org/10.3390/ijms22063108
Chicago/Turabian StyleKazimierczak, Urszula, Ewelina Dondajewska, Maria Zajaczkowska, Marianna Karwacka, Tomasz Kolenda, and Andrzej Mackiewicz. 2021. "LATS1 Is a Mediator of Melanogenesis in Response to Oxidative Stress and Regulator of Melanoma Growth" International Journal of Molecular Sciences 22, no. 6: 3108. https://doi.org/10.3390/ijms22063108