Preclinical Assessment of a New Hybrid Compound C11 Efficacy on Neurogenesis and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice
Abstract
:1. Introduction
2. Results
2.1. Protective Abilities of C11—In Vitro Studies
2.2. Cognitive Functions and Neurogenesis—In Vivo Studies
2.2.1. Evaluation of the Effects of Long-Term Administration of C11 and LEV on the Total Amount of Newborn Cells in the Dentate SGZ and Granular Cell Layer (GCL) of Mouse Hippocampus after PILO Induced SE
2.2.2. Evaluation of the Effects of Long-Term Administration of C11 and LEV on the Newborn Neurons in the Dentate SGZ and GCL of Mouse Hippocampus after PILO Induced SE
2.2.3. Evaluation of the Effects of Long-Term Administration of C11 and LEV on the Newborn Astrocytes in the Dentate SGZ and GCL of Mouse Hippocampus after PILO Induced SE
2.2.4. Evaluation of the Effects of Long-Term Administration of C11 and LEV on Mouse Spatial Learning and Memory after PILO Induced SE
2.2.5. The Effect of Long-Term Treatment with C11 and LEV on the Level of NAA/Cr, GABA/Cr, Glc/Cr, Glt/Cr, and Gln/Cr in Mouse Brain after PILO-Induced SE
3. Discussion
4. Materials and Methods
4.1. In Vitro Study
4.1.1. Reagents
4.1.2. Neuroblastoma Cell Line
4.1.3. Differentiation of SH-SY5Y towards Neuronal Cells
4.1.4. Astroglia Cell Culture
4.1.5. Cell Viability Assessment—MTT Assay
4.2. In Vivo Study
4.2.1. Animals and Experimental Conditions
4.2.2. Status Epilepticus (SE) in Mice
4.2.3. Drugs
4.2.4. Drugs Administration
- PILO C11,
- PILO LEV,
- PILO Control group (PILO + water for injections + Tween 80), and
- Healthy Control group (water for injections + Tween 80)
4.2.5. Behavioral Study-Spatial Learning and Memory (MWM Test)
4.2.6. Magnetic Resonance Spectroscopy (MRS)
4.2.7. Brain Slice Preparation
4.2.8. Immunohistochemical Staining-Neurogenesis
4.2.9. Confocal Microscopy and Cell Counting
4.3. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Andres-Mach, M.; Szewczyk, A.; Zagaja, M.; Szala-Rycaj, J.; Lemieszek, M.K.; Maj, M.; Abram, M.; Kaminski, K. Preclinical Assessment of a New Hybrid Compound C11 Efficacy on Neurogenesis and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice. Int. J. Mol. Sci. 2021, 22, 3240. https://doi.org/10.3390/ijms22063240
Andres-Mach M, Szewczyk A, Zagaja M, Szala-Rycaj J, Lemieszek MK, Maj M, Abram M, Kaminski K. Preclinical Assessment of a New Hybrid Compound C11 Efficacy on Neurogenesis and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice. International Journal of Molecular Sciences. 2021; 22(6):3240. https://doi.org/10.3390/ijms22063240
Chicago/Turabian StyleAndres-Mach, Marta, Aleksandra Szewczyk, Mirosław Zagaja, Joanna Szala-Rycaj, Marta Kinga Lemieszek, Maciej Maj, Michał Abram, and Krzysztof Kaminski. 2021. "Preclinical Assessment of a New Hybrid Compound C11 Efficacy on Neurogenesis and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice" International Journal of Molecular Sciences 22, no. 6: 3240. https://doi.org/10.3390/ijms22063240