Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA
Abstract
:1. Introduction
2. Results
2.1. Analysis of hRIDA Expression in Different Human Cell Lines and Post-Translational Modification
2.2. K-Succinylation of hRIDA Was Under the Control of SIRT5
2.3. K-Succinylation Negatively Affects Enzymatic Activity of hRIDA
2.4. In Silico Analysis of K-Succinylation Influence on hRIDA Structures
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Growth Curve Analysis
4.2. Isolation of RNA from Cultured Cells and Real-Time qPCR Analysis
4.3. Western Blotting Analysis and Immunoprecipitation
4.4. Plasmids and Recombinant Proteins Synthesis
4.5. RNA Interference Analysis
4.6. In Vitro hRIDA Desuccinylation and Succinylation Assays
4.7. hRIDA Enzymatic Assay
4.8. 3D Modeling and Docking Simulations
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Siculella, L.; Giannotti, L.; Di Chiara Stanca, B.; Calcagnile, M.; Rochira, A.; Stanca, E.; Alifano, P.; Damiano, F. Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA. Int. J. Mol. Sci. 2021, 22, 3804. https://doi.org/10.3390/ijms22083804
Siculella L, Giannotti L, Di Chiara Stanca B, Calcagnile M, Rochira A, Stanca E, Alifano P, Damiano F. Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA. International Journal of Molecular Sciences. 2021; 22(8):3804. https://doi.org/10.3390/ijms22083804
Chicago/Turabian StyleSiculella, Luisa, Laura Giannotti, Benedetta Di Chiara Stanca, Matteo Calcagnile, Alessio Rochira, Eleonora Stanca, Pietro Alifano, and Fabrizio Damiano. 2021. "Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA" International Journal of Molecular Sciences 22, no. 8: 3804. https://doi.org/10.3390/ijms22083804