TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts
Abstract
:1. Introduction
2. Results
2.1. TRPM7 Is Expressed in HAT-7 Cells and in Mouse Ameloblasts
2.2. HAT-7 Cells Exhibit TRPM7-Like Ion Currents
2.3. TRPM7 Activators Stimulate Ca2+ Influx in HAT-7 Cells
2.4. SOCE Blocker BTP2 Inhibits Ca2+ Influx Stimulated by Naltriben but Not Mibefradil
2.5. TRPM7 Currents and Ca2+ Influx Are pH Sensitive
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. RT-qPCR
4.3. Immunohistochemistry
4.4. Electrophysiology
4.5. Calcium Imaging
4.6. Intracellular pH Measurements
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kádár, K.; Juhász, V.; Földes, A.; Rácz, R.; Zhang, Y.; Löchli, H.; Kató, E.; Köles, L.; Steward, M.C.; DenBesten, P.; et al. TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts. Int. J. Mol. Sci. 2021, 22, 3992. https://doi.org/10.3390/ijms22083992
Kádár K, Juhász V, Földes A, Rácz R, Zhang Y, Löchli H, Kató E, Köles L, Steward MC, DenBesten P, et al. TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts. International Journal of Molecular Sciences. 2021; 22(8):3992. https://doi.org/10.3390/ijms22083992
Chicago/Turabian StyleKádár, Kristóf, Viktória Juhász, Anna Földes, Róbert Rácz, Yan Zhang, Heike Löchli, Erzsébet Kató, László Köles, Martin C. Steward, Pamela DenBesten, and et al. 2021. "TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts" International Journal of Molecular Sciences 22, no. 8: 3992. https://doi.org/10.3390/ijms22083992