Xanthohumol-Induced Rat Glioma C6 Cells Death by Triggering Mitochondrial Stress
Abstract
:1. Introduction
2. Results
2.1. XN Affected C6 Cell Proliferation in Concentration and Time-Dependent Way
2.2. XN Treatment-Induced AIF-Dependent Apoptosis in C6 Cells
2.3. XN Increased the Amount of Mitochondrial ROS in the Process of Cell Death
2.4. XN Affected Mitochondrial Metabolism in C6 Cells
2.5. XN Reduced Mitochondrial Membrane Potential (MMP) in C6 Cell
2.6. XN Caused Fusion and Fission Imbalance by Reducing the Mitochondrial Fusion Function in C6 Cells
2.7. XN-Induced Mitophagy in C6 Cell
2.8. LONP1 Alleviated the Injury of XN
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Cell Culture
4.3. Cell Viability Assay and Morphological Observation
4.4. Trypan Blue Assay
4.5. Cell Cycle Assay
4.6. Apoptosis Assay
4.7. Measurement of Mitochondrial ROS
4.8. Detection of the Mitochondrial Membrane Potential
4.9. Western Blot Analysis
4.10. Immunocytochemistry
4.11. Detection of ATP, ADP, and AMP
4.12. Detection of Content of Succinic Dehydrogenase
4.13. Immunofluorescence staining
4.14. Detection of Mitochondrial Abundance
4.15. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hou, S.; Song, Y.; Sun, D.; Zhu, S.; Wang, Z. Xanthohumol-Induced Rat Glioma C6 Cells Death by Triggering Mitochondrial Stress. Int. J. Mol. Sci. 2021, 22, 4506. https://doi.org/10.3390/ijms22094506
Hou S, Song Y, Sun D, Zhu S, Wang Z. Xanthohumol-Induced Rat Glioma C6 Cells Death by Triggering Mitochondrial Stress. International Journal of Molecular Sciences. 2021; 22(9):4506. https://doi.org/10.3390/ijms22094506
Chicago/Turabian StyleHou, Shaozhi, Yang Song, Di Sun, Shujun Zhu, and Zhenhua Wang. 2021. "Xanthohumol-Induced Rat Glioma C6 Cells Death by Triggering Mitochondrial Stress" International Journal of Molecular Sciences 22, no. 9: 4506. https://doi.org/10.3390/ijms22094506