Interactions between Phosphatidylcholine and Kaempferol or Myristicin: Langmuir Monolayers and Microelectrophoretic Studies
Abstract
:1. Introduction
2. Results and Discussion
2.1. Monolayer Experiment
2.2. Mixed Monolayer Experiment and Theoretical Consideration
2.2.1. Phosphatidylcholine–Kaempferol Complex
2.2.2. Phosphatidylcholine–Myricetin Complex
2.3. Microelectrophoretic Experiments
- -
- The isoelectric point for the PC lipid with kaempferol (e.g., for a 1:1 ratio) lies at lower pH values (~4.0) compared to the PC–M system (pH ~ 4.4);
- -
- The surface charge density values for the PC–K system in the range of pH 2–9 showed values from 3.0 to −4.1 × 10−2 C m−2, while for the PC–M system, these values were higher and were 3.7 × 10−2 C m−2 (for PC–M (ratio 1:1) at pH ~ 2) to −4.2 × 10−2 C m−2 (for pure M).
3. Materials and Methods
3.1. Materials
3.1.1. Monolayer Preparation—Spreading Solvent and Subphase
3.1.2. Liposomes Preparation
3.2. Monolayer Measurements
3.3. Microelectrophoretic Measurements
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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System | Surface Area of Complex(Å2 Molecule−1) | Stability Constant (m2∙mol−1) | Complex Formation Energy (kJ mol−1) |
---|---|---|---|
PC–K | 64 | 2.8 × 106 | −37 ± 1 |
PC–M | 60 | 2.1 × 106 | −36 ± 1 |
Examined System | Isoelectric Point | Surface Charge Density δ (10–2 C m–2) | |
---|---|---|---|
Low pH Values | High pH Values | ||
PC | ~3.2 | 0.8 ± 0.1 | −0.9 ± 0.1 |
PC–K (3:1) | ~4.1 | 2.8 ± 0.2 | −2.1 ± 0.3 |
PC–K (1:1) | ~4.0 | 2.4 ± 0.2 | −3.0 ± 0.3 |
PC–K (1:3) | ~3.4 | 1.1 ± 0.1 | −3.4 ± 0.5 |
K | ~2.0 | 0.1 ± 0.0 | −4.1 ± 0.6 |
Examined System | Isoelectric Point | Surface Charge Density δ (10−2 C m−2) | |
---|---|---|---|
Low pH Values | High pH Values | ||
PC | ~3.2 | 0.8 ± 0.1 | −0.9 ± 0.1 |
PC–M (3:1) | ~4.1 | 2.8 ± 0.6 | −1.4 ± 0.2 |
PC–M (1:1) | ~4.3 | 3.7 ± 0.4 | −1.8 ± 0.2 |
PC–M (1:3) | ~4.1 | 1.2 ± 0.2 | −2.3 ± 0.2 |
M | ~3.7 | 0.9 ± 0.2 | −4.2 ± 0.4 |
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Laszuk, P.; Petelska, A.D. Interactions between Phosphatidylcholine and Kaempferol or Myristicin: Langmuir Monolayers and Microelectrophoretic Studies. Int. J. Mol. Sci. 2021, 22, 4729. https://doi.org/10.3390/ijms22094729
Laszuk P, Petelska AD. Interactions between Phosphatidylcholine and Kaempferol or Myristicin: Langmuir Monolayers and Microelectrophoretic Studies. International Journal of Molecular Sciences. 2021; 22(9):4729. https://doi.org/10.3390/ijms22094729
Chicago/Turabian StyleLaszuk, Paulina, and Aneta D. Petelska. 2021. "Interactions between Phosphatidylcholine and Kaempferol or Myristicin: Langmuir Monolayers and Microelectrophoretic Studies" International Journal of Molecular Sciences 22, no. 9: 4729. https://doi.org/10.3390/ijms22094729