Molecular Basis of Multiple Mitochondrial Dysfunctions Syndrome 2 Caused by CYS59TYR BOLA3 Mutation
Abstract
:1. Introduction
2. Results
2.1. Cys59Tyr Mutation Structurally Perturbed Only the Fe–S Cluster-Binding Region of BOLA3
2.2. Cys59Tyr BOLA3 Mutation Modified the Interaction of BOLA3 with Apo GLRX5
2.3. C59Y BOLA3–GLRX5 Complex Bound a [2Fe–2S]2+ Cluster
3. Discussion
4. Materials and Methods
4.1. Protein Expression and Purification
4.2. In Vitro Chemical Reconstitution of the Hetero-Complexes
4.3. NMR Spectroscopy to Characterize Proteins and Hetero-Complexes in Their Apo Forms
4.4. Spectroscopic Methods to Characterize Fe–S Cluster-Binding and Transfer
4.5. Data-Driven Biomolecular Docking
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Saudino, G.; Suraci, D.; Nasta, V.; Ciofi-Baffoni, S.; Banci, L. Molecular Basis of Multiple Mitochondrial Dysfunctions Syndrome 2 Caused by CYS59TYR BOLA3 Mutation. Int. J. Mol. Sci. 2021, 22, 4848. https://doi.org/10.3390/ijms22094848
Saudino G, Suraci D, Nasta V, Ciofi-Baffoni S, Banci L. Molecular Basis of Multiple Mitochondrial Dysfunctions Syndrome 2 Caused by CYS59TYR BOLA3 Mutation. International Journal of Molecular Sciences. 2021; 22(9):4848. https://doi.org/10.3390/ijms22094848
Chicago/Turabian StyleSaudino, Giovanni, Dafne Suraci, Veronica Nasta, Simone Ciofi-Baffoni, and Lucia Banci. 2021. "Molecular Basis of Multiple Mitochondrial Dysfunctions Syndrome 2 Caused by CYS59TYR BOLA3 Mutation" International Journal of Molecular Sciences 22, no. 9: 4848. https://doi.org/10.3390/ijms22094848