Peer-Review Record
Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of Pseudomonas aeruginosa to Secondary Metabolites from Carnivorous Plants
Int. J. Mol. Sci. 2021, 22(9), 4849; https://doi.org/10.3390/ijms22094849
by
Marta Krychowiak-Maśnicka 1,*, Mirosława Krauze-Baranowska 2, Sylwia Godlewska 2, Zbigniew Kaczyński 3
, Aleksandra Bielicka-Giełdoń 3, Natalia Grzegorczyk 1, Magdalena Narajczyk 4, Joanna E. Frackowiak 5 and Aleksandra Krolicka 1,*
, Aleksandra Bielicka-Giełdoń 3, Natalia Grzegorczyk 1, Magdalena Narajczyk 4, Joanna E. Frackowiak 5 and Aleksandra Krolicka 1,*Reviewer 1:
Anonymous
Reviewer 2:
Anonymous
Int. J. Mol. Sci. 2021, 22(9), 4849; https://doi.org/10.3390/ijms22094849
Submission received: 17 March 2021
/
Accepted: 29 April 2021
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Published: 3 May 2021
(This article belongs to the Special Issue Development of Active Compounds to Combat Antibiotic Resistant Microorganisms)
Round 1
Reviewer 1 Report
- Authors present an original study. It is well presented that carnivorous plants are natural sources of secondary metabolites with biological activity. Tissues of three carnivorous plants species, i.e. D. binata, D. muscipula and D. gigantea, were obtained under in vitro conditions. Introduction provides appropriate background with relevant literature review. Research design is appropriate. Methods used are well presented and results are clearly given. Authors confirmed that P. aeruginosa is resistant to abroad range of secondary metabolites from carnivorous plants, i.e. naphthoquinones, flavonoids, phenolic acids (MBC = 512 µg mL-1) and only weakly sensitive to their mixtures, i.e. extracts and extracts’ fractions.
- Study indicates the significant potential of nanoparticles to modulate the activity of selected secondary metabolites and revisit their antimicrobial potential towards human pathogenic bacteria. The synergy of nanoparticles and extracts is pronounced when their mixture is used towards P. aeruginosa in comparison to S. aureus.
- On my opinion manuscript should be accepted in present form.
Reviewer 2 Report
The article is about increasing the antimicrobial activity of secondary metabolites using silver nanoparticles, which is novel and important at present. The structure of the text is adequate, and allows the identification of ideas easily. In order to improve the understanding, the abbreviations (MBC, FBCI) should be defined at the beginning of the article, where they are used for the first time.