PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
Abstract
:1. Introduction
2. Results
2.1. Platyphyllenone Does Not Affect the Viability of Human Oral Cancer Cells
2.2. Platyphyllenone Inhibits the Motility of Human Oral Cancer Cells
2.3. Platyphyllenone Inhibits Migration and Invasion of Human Oral Cancer Cells
2.4. Platyphyllenone Reduces Motility of Human Oral Cancer Cells by Modulating p38 Pathway
2.5. Platyphyllenonealters Expression of Epithelial–Mesenchymal Transition Proteins
2.6. Platyphyllenone Alters the Expression of Cathepsin L in Human Oral Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Compound
4.3. MTT Assay
4.4. In Vitro Wound Closure
4.5. Cell Migration and Invasion Assay
4.6. Western Blot Assay
4.7. Transfection Assay
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bharath Kumar, V.; Lin, J.-T.; Mahalakshmi, B.; Chuang, Y.-C.; Ho, H.-Y.; Lin, C.-C.; Lo, Y.-S.; Hsieh, M.-J.; Chen, M.-K. PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition. Int. J. Mol. Sci. 2021, 22, 5012. https://doi.org/10.3390/ijms22095012
Bharath Kumar V, Lin J-T, Mahalakshmi B, Chuang Y-C, Ho H-Y, Lin C-C, Lo Y-S, Hsieh M-J, Chen M-K. PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition. International Journal of Molecular Sciences. 2021; 22(9):5012. https://doi.org/10.3390/ijms22095012
Chicago/Turabian StyleBharath Kumar, V., Jen-Tsun Lin, B. Mahalakshmi, Yi-Ching Chuang, Hsin-Yu Ho, Chia-Chieh Lin, Yu-Sheng Lo, Ming-Ju Hsieh, and Mu-Kuan Chen. 2021. "PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition" International Journal of Molecular Sciences 22, no. 9: 5012. https://doi.org/10.3390/ijms22095012