In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach
, Bruna dos Santos Rodrigues †, Alanah Pieters, Anne Caufriez, Kaat Leroy, Raf Van Campenhout, Axelle Cooreman, Ana Rita Gomes, Emma Arnesdotter, Eva Gijbels and Mathieu Vinken *Round 1
Reviewer 1 Report
Review of “In vitro liver toxicity testing of chemicals: a pragmatic approach”, by Tabernilla et al.
This work consists in a review of the different in vitro approaches and assays to evaluate the cytotoxicity and hepatotoxicity of chemicals. A detailed explanation is provided for each assay, and their advantages and disadvantages are discussed.
I have found this paper really interesting, easy to read and understand, and the conclusions/results are really well founded. The description of the different assays is very complete and well referenced, and in my opinion, this piece of work can be very helpful to people having to decide between different assay options.
I strongly recommend the publication of this review as it is, without significant modifications. The only remark to authors is to pay attention to the format, the figures are not of the highest quality, and the text in tables 1 and 2 should be justified.
Author Response
Reviewer #1: The only remark to authors is to pay attention to the format, the figures are not of the highest quality, and the text in Tables 1 and 2 should be justified.
Authors: Following the recommendation of the Reviewer 1, the quality of the figures has been increased and the text in Tables 1 and 2 has been justified.
Reviewer 2 Report
Tabernilla et al. provided a comprehensive review on in vitro liver toxicity testing of chemicals. They also proposed a rationalized two-tire in vitro testing strategy to detect liver toxicity triggered by chemicals and provided the most commonly used in vitro assays that can be used in both tiers with advantages and disadvantages.
Overall, this is an important review summarizing all the in vitro liver toxicity testing of chemicals which will be of great interest to the readers of this journal. The paper is well-organized and well-written. I applause the authors for their efforts to beautifully summarizing the assays. It took me some time to finish the paper and I did not find any major concerns. Bullet points can be removed or be left-aligned from Tables 1 and 2.
Author Response
Reviewer #2: Bullet points can be removed or be left-aligned from Tables 1 and 2.
Authors: Following the recommendation of the Reviewer 2, the bullet points from Table 1 and 2 have been left-aligned.
