CD47 Binding on Vascular Endothelial Cells Inhibits IL-17-Mediated Leukocyte Adhesion
Abstract
:1. Introduction
2. Results
2.1. Thrombospondin-1-Derived CD47-Binding Peptide Inhibits Vascular Leukocyte Adhesion in Chronic Uveitis
2.2. The Lack of TSP-1 Prevents Retinal Leukocyte Adherence in Acute Uveitis Inflammation
2.3. Increased VCAM-1 Expression and Leukocyte Adhesion in Retinal Vessels of TSP-1-Deficient Mice
2.4. IL-17 Induces VCAM-1 Expression in Endothelial Cells Independently of Their Endogenous TSP-1
2.5. IL-17-Induced VCAM-1 Expression on Vascular Endothelial Cells and Leukocyte Adhesion Are Inhibited by CD47 Ligation
2.6. Endogenous TSP-1 Expressed by Vascular Endothelial Cells Mediates Increased VCAM-1 Expression via CD36 Binding
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Antibodies and Reagents
4.3. Primary Cultures of Murine Vascular Endothelial Cells
4.4. Immunostaining and Analysis
4.5. Leukocyte–Endothelial Cell Adhesion Assay
4.6. Experimental Autoimmune Uveitis (EAU) Model—Peptide Treatment and Disease Monitoring
4.7. Quantification of Adherent Leukocytes in Retinal Vessels
4.8. Real-Time PCR
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Soriano-Romaní, L.; Mir, F.A.; Singh, N.; Chin, I.; Hafezi-Moghadam, A.; Masli, S. CD47 Binding on Vascular Endothelial Cells Inhibits IL-17-Mediated Leukocyte Adhesion. Int. J. Mol. Sci. 2022, 23, 5705. https://doi.org/10.3390/ijms23105705
Soriano-Romaní L, Mir FA, Singh N, Chin I, Hafezi-Moghadam A, Masli S. CD47 Binding on Vascular Endothelial Cells Inhibits IL-17-Mediated Leukocyte Adhesion. International Journal of Molecular Sciences. 2022; 23(10):5705. https://doi.org/10.3390/ijms23105705
Chicago/Turabian StyleSoriano-Romaní, Laura, Fayaz A. Mir, Niharika Singh, Ian Chin, Ali Hafezi-Moghadam, and Sharmila Masli. 2022. "CD47 Binding on Vascular Endothelial Cells Inhibits IL-17-Mediated Leukocyte Adhesion" International Journal of Molecular Sciences 23, no. 10: 5705. https://doi.org/10.3390/ijms23105705