Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Ti2CTx MXene
2.2. Biological Response to Ti2CTx Nanosheet
3. Experimental Design
3.1. Materials
3.2. Synthesis Procedure of Ti2CTx MXenes
3.3. Characterization of Nanosheets
3.4. Biological Activity
3.4.1. hWJ-MSCs Cell Culture
3.4.2. Scratch Assay
3.4.3. MTT Assay
3.4.4. Cell Viability Assessment via Staining with Propidium Iodide
3.4.5. Total ROS Production
3.4.6. Mitochondrial Superoxide Detection via MitoSOX-Red Indicator
3.4.7. Stemness and Differentiation Potential
RT-PCR Analysis
Tri-Lineage Differentiation of MSCs
3.4.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Singh, S.K.; Singh, A.; Kumar, V.; Gupta, J.; Umrao, S.; Kumar, M.; Sarma, D.K.; Leja, M.; Bhandari, M.P.; Verma, V. Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells. Int. J. Mol. Sci. 2022, 23, 5816. https://doi.org/10.3390/ijms23105816
Singh SK, Singh A, Kumar V, Gupta J, Umrao S, Kumar M, Sarma DK, Leja M, Bhandari MP, Verma V. Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2022; 23(10):5816. https://doi.org/10.3390/ijms23105816
Chicago/Turabian StyleSingh, Suraj Kumar, Anshuman Singh, Vinod Kumar, Jalaj Gupta, Sima Umrao, Manoj Kumar, Devojit Kumar Sarma, Marcis Leja, Manohar Prasad Bhandari, and Vinod Verma. 2022. "Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells" International Journal of Molecular Sciences 23, no. 10: 5816. https://doi.org/10.3390/ijms23105816