Structural Aspects of E. coli Type II Asparaginase in Complex with Its Secondary Product L-Glutamate
Abstract
:1. Introduction
2. Results
2.1. Overall Apo-N24S Structure
2.2. Overall N24S–GLU Structure
2.3. Ligand and Active Site Residues Displacement
3. Discussion
4. Materials and Methods
4.1. Protein Production and Crystallization
4.2. Data Collection and Refinement
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Structure | PDB ID | Property | Value |
---|---|---|---|
apo-N24S | 7R57 | Data collection | |
Space group | C2 | ||
Cell constants (a, b, c) | 152.14 Å, 62.44 Å, 143.41 Å | ||
Cell constants (α, β, γ) | 90.00°, 118.19°, 90.00° | ||
Resolution | 47.09–1.40 Å | ||
Rmerge | 0.05 | ||
I/σI | 1.4 | ||
Completeness in resolution range | 98.5% | ||
Multiplicity | 2.8 | ||
Refinement | |||
Resolution range | 47.09–1.70 Å | ||
No. of reflections | 128,815 | ||
Rwork/Rfree | 0.17/0.19 | ||
R.m.s. bond length | 0.01 Å | ||
R.m.s. bond angles | 1.02° | ||
N24S–GLU | 7R5Q | Data collection | |
Space group | C2 | ||
Cell constants (a, b, c) | 151.03 Å, 61.95 Å, 142.56 Å | ||
Cell constants (α, β, γ) | 90.00°, 118.31°, 90.00° | ||
Resolution | 47.81–1.57 Å | ||
Rmerge | 0.07 | ||
I/σI | 1.26 | ||
Completeness in resolution range | 99.3 | ||
Multiplicity | 2.7 | ||
Refinement | |||
Resolution range | 47.811–1.90 Å | ||
No. of reflections | 90,818 | ||
Rwork/Rfree | 0.17/0.20 | ||
R.m.s. bond length | 0.0079 Å | ||
R.m.s. bond angles | 0.93° |
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Maggi, M.; Scotti, C. Structural Aspects of E. coli Type II Asparaginase in Complex with Its Secondary Product L-Glutamate. Int. J. Mol. Sci. 2022, 23, 5942. https://doi.org/10.3390/ijms23115942
Maggi M, Scotti C. Structural Aspects of E. coli Type II Asparaginase in Complex with Its Secondary Product L-Glutamate. International Journal of Molecular Sciences. 2022; 23(11):5942. https://doi.org/10.3390/ijms23115942
Chicago/Turabian StyleMaggi, Maristella, and Claudia Scotti. 2022. "Structural Aspects of E. coli Type II Asparaginase in Complex with Its Secondary Product L-Glutamate" International Journal of Molecular Sciences 23, no. 11: 5942. https://doi.org/10.3390/ijms23115942