Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet
Abstract
:1. Introduction
2. Results
2.1. Broomcorn Millet Varieties Exhibited Great Differences in Response to Cd Stress
2.2. Microstructural Difference of the Two Broomcorn Millet Varieties under Cd Stress
2.3. Root Physiological Differences in Response to Cd Stress
2.4. Overview of Transcriptome Sequencing Results
2.5. Differentially Expressed Genes and Their Dynamic Responses to Cd Stress
2.6. Identification of Hub Modules through WGCNA
2.7. GO and KEGG Enrichment Analysis of DEGs in Hub Modules
2.8. DEGs Involved in Phenylpropanoid Biosynthesis and GSH Metabolism Pathway
2.9. Transporter Genes Involved in Cd Uptake and Translocation
3. Discussion
4. Materials and Methods
4.1. Transporter Genes Involved in Cd Uptake and Translocation
4.2. Determination of Phenotypic and Cd-Related Traits
4.3. Determination of Physiological Parameters
4.4. Microstructural Observations of Root Tissu
4.5. Transcriptome Sequencing and Analysis
4.6. Quantitative Real-Time PCR Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, J.; Zhang, D.; Zhang, Y.; Zhou, H.; Chen, P.; Yuan, Y.; Yang, Q.; Zhao, L.; Feng, B. Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet. Int. J. Mol. Sci. 2022, 23, 6148. https://doi.org/10.3390/ijms23116148
Liu J, Zhang D, Zhang Y, Zhou H, Chen P, Yuan Y, Yang Q, Zhao L, Feng B. Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet. International Journal of Molecular Sciences. 2022; 23(11):6148. https://doi.org/10.3390/ijms23116148
Chicago/Turabian StyleLiu, Jiajia, Dazhong Zhang, Yuanbo Zhang, Hao Zhou, Pengliang Chen, Yuhao Yuan, Qinghua Yang, Lin Zhao, and Baili Feng. 2022. "Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet" International Journal of Molecular Sciences 23, no. 11: 6148. https://doi.org/10.3390/ijms23116148