Genome-Wide Identification of Long Noncoding RNA and Their Potential Interactors in ISWI Mutants
Abstract
:1. Introduction
2. Results
2.1. Hybridization and Identification of ISWI Mutation Lines
2.2. Identification and Genomic Characteristics of lncRNAs and mRNAs
2.3. LncRNAs Expression Changes Significantly in ISWI-Mutated Background
2.4. Differentially Expressed mRNAs and lncRNAs
2.5. Potential Interactors of DE-lncRNAs
2.6. Differentially Expressed Transposons
3. Discussion
4. Materials and Methods
4.1. Drosophila Stocks and Crosses
4.2. Immunostaining of Chromosomes
4.3. Embryo TSA-FISH
4.4. RNA Extraction and Sequencing
4.5. RNA-Seq Reads Mapping and Transcriptome Assembly
4.6. Transcripts Filtering and Prediction
4.7. Comparisons between lncRNAs and mRNA Transcripts
4.8. Ratio Distribution
4.9. Differential Expression Analysis
4.10. Potential Reactors of DE-lncRNA
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, L.; Zhang, S.; Wang, R.; Sun, L. Genome-Wide Identification of Long Noncoding RNA and Their Potential Interactors in ISWI Mutants. Int. J. Mol. Sci. 2022, 23, 6247. https://doi.org/10.3390/ijms23116247
Zhang L, Zhang S, Wang R, Sun L. Genome-Wide Identification of Long Noncoding RNA and Their Potential Interactors in ISWI Mutants. International Journal of Molecular Sciences. 2022; 23(11):6247. https://doi.org/10.3390/ijms23116247
Chicago/Turabian StyleZhang, Ludan, Shuai Zhang, Ruixue Wang, and Lin Sun. 2022. "Genome-Wide Identification of Long Noncoding RNA and Their Potential Interactors in ISWI Mutants" International Journal of Molecular Sciences 23, no. 11: 6247. https://doi.org/10.3390/ijms23116247