A Multifunctional and Fast-Response Lysosome-Targetable Fluorescent Probe for Monitoring pH and Isoxaflutole
Abstract
:1. Introduction
2. Results and Discussion
2.1. Design and Synthesis of MMN
2.2. pH-Dependent Sensing Performance
2.3. pKa Value, Reversibility and Photostability
2.4. Recognition Mechanism of MMN to H+
2.5. Colocalization Imaging Experiment and Test Stri
2.6. Detection of Isoxaflutole
3. Experimental Section
3.1. Materials and Methods
3.2. Synthesis
3.2.1. Synthesis of 4-Morpholine-1,8-naphthalic Anhydride (1)
3.2.2. Synthesis of N-Carboxymethyl-4-morpholine-1,8-naphthalimide (2)
3.2.3. Synthesis of N-(2-Morpholinoethyl)acetamide-4-morpholine-1,8-naphthimide (MMN)
3.3. General Method for the Spectra Experiments of MMN
3.4. Cell Incubation and Fluorescence Imaging
3.5. Computational Methods
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Type | Working Media | Sensitive Range (pH) | Sensing Mechanism | Application | References |
---|---|---|---|---|---|
Off–on | MeCN/H2O (9/1) | 4.2–6.0 | ICT | Paper strip/Targetable Imaging/pesticide detection | MMN |
On–off | PBS buffer | 5.0–7.2 | PET | Cell imaging | [28] |
Off–on–off | H2O | 2.0–10 | PET | - | [29] |
Colorimetric probe | DMSO | 4.5–8.0 | FRET | Cell imaging | [30] |
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Yang, L.; Liu, Y.; Yue, M.; Li, P.; Liu, Y.; Ye, F.; Fu, Y. A Multifunctional and Fast-Response Lysosome-Targetable Fluorescent Probe for Monitoring pH and Isoxaflutole. Int. J. Mol. Sci. 2022, 23, 6256. https://doi.org/10.3390/ijms23116256
Yang L, Liu Y, Yue M, Li P, Liu Y, Ye F, Fu Y. A Multifunctional and Fast-Response Lysosome-Targetable Fluorescent Probe for Monitoring pH and Isoxaflutole. International Journal of Molecular Sciences. 2022; 23(11):6256. https://doi.org/10.3390/ijms23116256
Chicago/Turabian StyleYang, Liu, Yan Liu, Mingli Yue, Ping Li, Yulong Liu, Fei Ye, and Ying Fu. 2022. "A Multifunctional and Fast-Response Lysosome-Targetable Fluorescent Probe for Monitoring pH and Isoxaflutole" International Journal of Molecular Sciences 23, no. 11: 6256. https://doi.org/10.3390/ijms23116256