Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging
Abstract
:1. Introduction
2. Results
2.1. TMT Identification of Differentially Regulated Proteins
2.2. GO Analysis and Annotation of Differentially Regulated Proteins
2.3. Significantly Upregulated Proteins and Pathway Analysis in 3dwb
2.4. Significantly Upregulated Proteins and Pathway Analysis in 12dwb
2.5. Significantly Downregulated Proteins and Pathway Analysis in 3dwb
2.6. Significantly Downregulated Proteins and Pathway Analysis in 12dwb
2.7. Differentially Regulated Proteins Common to Both 3dwb and 12dwb
2.8. Protein-Protein Interaction (PPI) Analysis
2.9. Validation of TMT Data with Real-Time qPCR
3. Discussion
4. Materials and Methods
4.1. Microorganism and Culture Conditions
4.2. Protein Extraction and Fractionation
4.3. Protein Reduction, Alkylation, and Digestion
4.4. TMT Labeling and High pH Fractionation
4.5. Nanoflow LC-ESI-MS/MS
4.5.1. Nanoflow LC-ESI-MS/MS Using Orbitrap Elite
4.5.2. Nanoflow LC-ESI-MS/MS Using Q Exactive
4.6. Database Search, Statistical Analysis, and Bioinformatics
4.7. Validation of TMT Data with qPCR Results
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rahman, M.A.; Amirkhani, A.; Chowdhury, D.; Mempin, M.; Molloy, M.P.; Deva, A.K.; Vickery, K.; Hu, H. Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging. Int. J. Mol. Sci. 2022, 23, 6415. https://doi.org/10.3390/ijms23126415
Rahman MA, Amirkhani A, Chowdhury D, Mempin M, Molloy MP, Deva AK, Vickery K, Hu H. Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging. International Journal of Molecular Sciences. 2022; 23(12):6415. https://doi.org/10.3390/ijms23126415
Chicago/Turabian StyleRahman, Md. Arifur, Ardeshir Amirkhani, Durdana Chowdhury, Maria Mempin, Mark P. Molloy, Anand Kumar Deva, Karen Vickery, and Honghua Hu. 2022. "Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging" International Journal of Molecular Sciences 23, no. 12: 6415. https://doi.org/10.3390/ijms23126415