Mutation of an Essential 60S Ribosome Assembly Factor MIDASIN 1 Induces Early Flowering in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Quantification of Flowering Time
2.3. RNA Sequencing and RNA-seq Data Analysis
2.4. Analysis of Transcript Abundance
3. Results
3.1. Mutation in MDN1 Leads to Early Flowering
3.2. Multiple Flowering Pathways Are Influenced in mdn1-1
3.3. Downregulation of FLC Is Associated with Early Flowering in mdn1-1
3.4. Photoperiod Pathway Contributes to mdn1-1 Early Flowering
3.5. ABI5 Contributes to Early Flowering in mdn1-1
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ABA | Abscisic acid |
ABI5 | ABSCISIC ACID-INSENSITIVE 5 |
CCA1 | CIRCADIAN CLOCK ASSOCIATED 1 |
CDF1 | CYCLING DOF FACTOR 1 |
CO | CONSTANTS |
CRYs | CRYPTOCHROMEs |
DAG | days after germination |
FCA | FLOWERING CONTROL LOCUS A |
FLC | FLOWERING LOCUS C |
FLD | FLOWERING LOCUS D |
FLK | FLOWERING LOCUS KH DOMAIN |
FRI | FRIGIDA |
FT | FLOWERING LOCUS T |
HFR1 | LONG HYPOCOTYL IN FAR-RED 1 |
LCL1 | LHY/CCA1-LIKE 1 |
LD | LUMINIDEPENDENS |
MDN1 | MIDASIN 1 |
PHYA | PHYTOCHROME A |
RBFs | ribosome biogenesis factors |
RPs | ribosomal proteins |
TOC1 | TIMING OF CAB OF EXPRESSION 1 |
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Li, K.; Wang, P.; Ding, T.; Hou, L.; Li, G.; Zhao, C.; Zhao, S.; Wang, X.; Li, P. Mutation of an Essential 60S Ribosome Assembly Factor MIDASIN 1 Induces Early Flowering in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 6509. https://doi.org/10.3390/ijms23126509
Li K, Wang P, Ding T, Hou L, Li G, Zhao C, Zhao S, Wang X, Li P. Mutation of an Essential 60S Ribosome Assembly Factor MIDASIN 1 Induces Early Flowering in Arabidopsis. International Journal of Molecular Sciences. 2022; 23(12):6509. https://doi.org/10.3390/ijms23126509
Chicago/Turabian StyleLi, Ke, Pengfei Wang, Tingting Ding, Lei Hou, Guanghui Li, Chuanzhi Zhao, Shuzhen Zhao, Xingjun Wang, and Pengcheng Li. 2022. "Mutation of an Essential 60S Ribosome Assembly Factor MIDASIN 1 Induces Early Flowering in Arabidopsis" International Journal of Molecular Sciences 23, no. 12: 6509. https://doi.org/10.3390/ijms23126509