DKK-1 Is Underexpressed in Mesenchymal Stem Cells from Patients with Ankylosing Spondylitis and Further Downregulated by IL-17
Abstract
:1. Introduction
2. Results
IL-17 Further Downregulates DKK-1 in MSCs from AS Patients
3. Discussion
4. Materials and Methods
4.1. Cell Isolation
4.2. In Vitro IL-17 Stimulation
4.3. Quantitative RT-PCR Analysis
4.4. Alkaline Phosphatase (ALP) Staining
4.5. Alizarin Red Staining
4.6. Von Kossa Staining
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Forward Primer | Reverse Primer |
---|---|---|
Runx2 | 5′-CGGAGTGGACGAGGCAAGA-3′ | 5′-GAGGCGGTCAGAGAACAAACT-3′ |
Osterix | 5′-AGGTTCCCCCAGCTCTCT-3′ | 5′-TTCTTTGTGCCTGCTTTGCC-3′ |
Osteonectin (sparc) | 5′-GGCAGAGGTGACTGAGGTATC-3′ | 5′-GTTTGCAGTGGTGGTTCTGG-3′ |
Axin2 | 5′-TGTGAGGTCCACGGAAACTG-3′ | 5′-TTCCATCTACACTGCTGTCCG-3′ |
Dkk1 | 5′-GAATAAGTACCAGACCATTGAC-3′ | 5′-CCATTTTTGCAGTAATTCCC-3′ |
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Daoussis, D.; Kanellou, A.; Panagiotopoulos, E.; Papachristou, D. DKK-1 Is Underexpressed in Mesenchymal Stem Cells from Patients with Ankylosing Spondylitis and Further Downregulated by IL-17. Int. J. Mol. Sci. 2022, 23, 6660. https://doi.org/10.3390/ijms23126660
Daoussis D, Kanellou A, Panagiotopoulos E, Papachristou D. DKK-1 Is Underexpressed in Mesenchymal Stem Cells from Patients with Ankylosing Spondylitis and Further Downregulated by IL-17. International Journal of Molecular Sciences. 2022; 23(12):6660. https://doi.org/10.3390/ijms23126660
Chicago/Turabian StyleDaoussis, Dimitrios, Anastasia Kanellou, Elias Panagiotopoulos, and Dionysios Papachristou. 2022. "DKK-1 Is Underexpressed in Mesenchymal Stem Cells from Patients with Ankylosing Spondylitis and Further Downregulated by IL-17" International Journal of Molecular Sciences 23, no. 12: 6660. https://doi.org/10.3390/ijms23126660