Use of Self-Assembled Colloidal Prodrug Nanoparticles for Controlled Drug Delivery of Anticancer, Antifibrotic and Antibacterial Mitomycin
Abstract
:1. Introduction
2. Results
2.1. Structural Characterization of the Synthesized Samples
2.2. Self-Assembled Particle Formation
2.3. Mucoadhesive Properties of Cysteamine Polymeric Conjugate
2.4. In Vitro Drug Release Measurement
2.5. In Vitro Evaluation of the Anticancer and Antibacterial Activity of MMC Samples
3. Discussion
4. Materials and Methods
4.1. Preparation of PVA-SA-CYS-MMC by One-Pot Reaction
4.2. Methods of Characterization
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | G′ (kPa) | G″ (kPa) | G* (kPa) |
---|---|---|---|
1% PVA-CYS | 24.7 | 7.55 | 32.25 |
1% Mucin | 12.4 | 0.78 | 13.18 |
1% PVA-CYS-Mucin | 158.3 | 23.2 | 181.5 |
Sample | 1st Day | 3rd Day | 5th Day | 7th Day | ||||
---|---|---|---|---|---|---|---|---|
Conc (mg/mL) | IC50 (mg/mL) | Conc (mg/mL) | IC50 (mg/mL) | Conc (mg/mL) | IC50 (mg/mL) | Conc (mg/mL) | IC50 (mg/mL) | |
Pure MMC | 0.254 | 0.029 ± 0.001 | 0.270 | 0.041 ± 0.002 | 0.273 | 0.026 ± 0.006 | 0.275 | 0.05 ± 0.045 |
Control | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
PVA-MMC (3%) | 0.063 | 0.014 ± 0.001 | 0.140 | 0.022 ± 0.007 | 0.173 | 0.043 ± 0.025 | 0.188 | 0.054 ± 0.029 |
PVA-MMC (21%) | 0.029 | 0.037 ± 0.004 | 0.065 | 0.038 ± 0.01 | 0.089 | 0.039 ± 0.007 | 0.097 | 0.029 ± 0.004 |
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Abdelghafour, M.M.; Deák, Á.; Szabó, D.; Dékány, I.; Rovó, L.; Janovák, L. Use of Self-Assembled Colloidal Prodrug Nanoparticles for Controlled Drug Delivery of Anticancer, Antifibrotic and Antibacterial Mitomycin. Int. J. Mol. Sci. 2022, 23, 6807. https://doi.org/10.3390/ijms23126807
Abdelghafour MM, Deák Á, Szabó D, Dékány I, Rovó L, Janovák L. Use of Self-Assembled Colloidal Prodrug Nanoparticles for Controlled Drug Delivery of Anticancer, Antifibrotic and Antibacterial Mitomycin. International Journal of Molecular Sciences. 2022; 23(12):6807. https://doi.org/10.3390/ijms23126807
Chicago/Turabian StyleAbdelghafour, Mohamed M., Ágota Deák, Diána Szabó, Imre Dékány, László Rovó, and László Janovák. 2022. "Use of Self-Assembled Colloidal Prodrug Nanoparticles for Controlled Drug Delivery of Anticancer, Antifibrotic and Antibacterial Mitomycin" International Journal of Molecular Sciences 23, no. 12: 6807. https://doi.org/10.3390/ijms23126807