Genome-Wide Identification of the Salvia miltiorrhiza SmCIPK Gene Family and Revealing the Salt Resistance Characteristic of SmCIPK13
Abstract
:1. Introduction
2. Result
2.1. Determination and Sequence Characterization of SmCIPKs Gene Family Members
2.2. Phylogenetic and Sequence Analysis of SmCIPKs
2.3. Expression Profiles of SmCIPK Genes
2.4. Localization of SmCIPK13 Protein and Analysis of Its Response to Abiotic Stresses
2.5. Expression and Overexpression of SmCIPK13 Promoted Salt Resistance in Yeast and Arabidopsis, Respectively
3. Discussion
4. Material and Methods
4.1. Plant Materials and Treatment
4.2. Identification and Characterization of Gene Family Members
4.3. Protein Sequence, Phylogenetic Tree Construction and Gene Structure Analysis
4.4. Analysis of SmCIPKs Expression Patterns in Different Tissues and under Different Treatments
4.5. Plasmid Construction, Transgenic Arabidopsis and Stress Treatment
4.6. Subcellular Localization of SmCIPK13 and Salt Stress Tolerance Assay in Yeast
4.7. Statistics Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID | CDS | AA | pI | Mw (Da) | Subcellular Localization |
---|---|---|---|---|---|---|
SmCIPK1 | SMil_00000703 | 1263 | 420 | 9.29 | 47,270.22 | Cytoplasm |
SmCIPK2 | SMil_00000704 | 1329 | 442 | 8.81 | 49,427.94 | Cytoplasm |
SmCIPK3 | SMil_00000853 | 1425 | 474 | 8.56 | 53,056.17 | Cytoplasm |
SmCIPK4 | SMil_00001122 | 1311 | 436 | 9.28 | 49,667.43 | Cytoplasm |
SmCIPK5 | SMil_00003013 | 1323 | 440 | 9.02 | 49,944.58 | Cytoplasm Nucleus |
SmCIPK6 | SMil_00003083 | 1311 | 436 | 9.25 | 49,042.18 | Cytoplasm |
SmCIPK7 | SMil_00003987 | 1128 | 375 | 5.99 | 42,844.14 | Cytoplasm |
SmCIPK8 | SMil_00004681 | 1425 | 474 | 8.88 | 53,641.64 | Cytoplasm Nucleus |
SmCIPK9 | SMil_00008243 | 1380 | 459 | 8.97 | 51,920.95 | Centrosome Cytoplasm Nucleus |
SmCIPK10 | SMil_00009044 | 1251 | 416 | 9.07 | 46,960.19 | Cytoplasm |
SmCIPK11 | SMil_00010169 | 1419 | 472 | 5.69 | 54,055.99 | Centrosome Cytoplasm Nucleus |
SmCIPK12 | SMil_00012715 | 1167 | 388 | 8.15 | 43,862.71 | Cytoplasm |
SmCIPK13 | SMil_00014175 | 1368 | 455 | 9.14 | 51,091.17 | Cytoplasm |
SmCIPK14 | SMil_00021346 | 1236 | 411 | 6.51 | 46,210.19 | Cytoplasm |
SmCIPK15 | SMil_00025018 | 1176 | 391 | 5.52 | 44,490.61 | Cytoplasm |
SmCIPK16 | SMil_00025262 | 1524 | 507 | 9.56 | 57,920.08 | Centrosome Cytoplasm Nucleus |
SmCIPK17 | SMil_00026858 | 1380 | 459 | 6.01 | 51,419.66 | Cytoplasm |
SmCIPK18 | SMil_00026916 | 1263 | 193 | 6.21 | 47,926.96 | Cytoplasm |
SmCIPK19 | SMil_00027660 | 1314 | 437 | 9.04 | 49,288.96 | Cytoplasm |
SmCIPK20 | SMil_00028555 | 1257 | 418 | 8.92 | 47,519.09 | Cytoplasm |
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Wang, S.; Li, Q. Genome-Wide Identification of the Salvia miltiorrhiza SmCIPK Gene Family and Revealing the Salt Resistance Characteristic of SmCIPK13. Int. J. Mol. Sci. 2022, 23, 6861. https://doi.org/10.3390/ijms23126861
Wang S, Li Q. Genome-Wide Identification of the Salvia miltiorrhiza SmCIPK Gene Family and Revealing the Salt Resistance Characteristic of SmCIPK13. International Journal of Molecular Sciences. 2022; 23(12):6861. https://doi.org/10.3390/ijms23126861
Chicago/Turabian StyleWang, Shuang, and Qi Li. 2022. "Genome-Wide Identification of the Salvia miltiorrhiza SmCIPK Gene Family and Revealing the Salt Resistance Characteristic of SmCIPK13" International Journal of Molecular Sciences 23, no. 12: 6861. https://doi.org/10.3390/ijms23126861