Identification and Fine Mapping of the Candidate Gene Controlling Multi-Inflorescence in Brassica napus
Abstract
:1. Introduction
2. Results
2.1. Morphological of Multi-Inflorescence and Possibility of Yield Increase
2.2. Fine Mapping of BnaMI Gene
2.3. Candidate Gene Analysis in the Mapping Region
2.4. An Identified 8.5 kb Insertion by Comparative Sequencing
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Population Development, and Trait Evaluation
4.2. Histological Analysis
4.3. Genomic DNA Extraction and Marker Development
4.4. Sequencing and functions prediction of candidate genes
4.5. RNA Extraction and Quantitative Real Time PCR (qRT−PCR)
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Number | Arabidopsis Homologous Gene Number | Annotations Information |
---|---|---|
BnaA05g08870D | AT2G35035.1 | urease accessory protein D |
BnaA05g08880D | AT2G35030.1 | Pentatricopeptide repeat (PPR) superfamily protein |
BnaA05g08890D | AT2G35020.1 | N-acetylglucosamine-1-phosphate uridylyltransferase 2 |
BnaA05g08900D | AT2G35000.1 | RING/U-box superfamily protein |
BnaA05g08910D | AT2G34990.1 | RING/U-box superfamily protein |
BnaA05g08950D | AT2G34930.1 | disease resistance family protein/LRR family protein |
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Lu, H.; Wu, H.; Zhu, G.; Yin, C.; Zhao, L.; Wen, J.; Yi, B.; Ma, C.; Tu, J.; Fu, T.; et al. Identification and Fine Mapping of the Candidate Gene Controlling Multi-Inflorescence in Brassica napus. Int. J. Mol. Sci. 2022, 23, 7244. https://doi.org/10.3390/ijms23137244
Lu H, Wu H, Zhu G, Yin C, Zhao L, Wen J, Yi B, Ma C, Tu J, Fu T, et al. Identification and Fine Mapping of the Candidate Gene Controlling Multi-Inflorescence in Brassica napus. International Journal of Molecular Sciences. 2022; 23(13):7244. https://doi.org/10.3390/ijms23137244
Chicago/Turabian StyleLu, Hongchen, Hanfei Wu, Guangfeng Zhu, Caijun Yin, Lun Zhao, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu, and et al. 2022. "Identification and Fine Mapping of the Candidate Gene Controlling Multi-Inflorescence in Brassica napus" International Journal of Molecular Sciences 23, no. 13: 7244. https://doi.org/10.3390/ijms23137244