Methuosis Contributes to Jaspine-B-Induced Cell Death
Abstract
:1. Introduction
2. Results
2.1. JB Induces Cytoplasmic Vacuolization in A549 and MCF7 Cells
2.2. JB-Induced Cytotoxicity Involves Methuosis
2.3. JB Activates AMPK
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Jaspine B and Jaspine B-Bodipy Synthesis
4.3. Cell Lines
4.4. Cell Viability
4.5. Annexin V-FITC Staining
4.6. Uptake of Fluid-Phase Tracer LY
4.7. Western Blotting
4.8. Phase Contrast and Confocal Microscopy
4.9. Transmission Electron Microscopy
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bielsa, N.; Casasampere, M.; Abad, J.L.; Enrich, C.; Delgado, A.; Fabriàs, G.; Lizcano, J.M.; Casas, J. Methuosis Contributes to Jaspine-B-Induced Cell Death. Int. J. Mol. Sci. 2022, 23, 7257. https://doi.org/10.3390/ijms23137257
Bielsa N, Casasampere M, Abad JL, Enrich C, Delgado A, Fabriàs G, Lizcano JM, Casas J. Methuosis Contributes to Jaspine-B-Induced Cell Death. International Journal of Molecular Sciences. 2022; 23(13):7257. https://doi.org/10.3390/ijms23137257
Chicago/Turabian StyleBielsa, Núria, Mireia Casasampere, Jose Luis Abad, Carlos Enrich, Antonio Delgado, Gemma Fabriàs, Jose M. Lizcano, and Josefina Casas. 2022. "Methuosis Contributes to Jaspine-B-Induced Cell Death" International Journal of Molecular Sciences 23, no. 13: 7257. https://doi.org/10.3390/ijms23137257