Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei
Abstract
:1. Introduction
2. Results
2.1. Analysis of T. bernacchii pIgR Gene Locus
2.2. Analysis of T. bernacchii pIgR cDNA
2.3. Analysis of T. bernacchii pIgR-Deduced Amino Acid Sequence
2.4. Structural Analysis of T. bernacchii pIgR
2.5. Basal Expression Analysis of pIgR Transcripts in T. bernacchii Mucosal Tissues and Lymphoid Organs
2.6. pIgR Expressing Cells in T. bernacchii Intestinal and Hepatic Tissues
3. Discussion
4. Materials and Methods
4.1. Biological Samples
4.2. Cloning of pIgR Transcript
4.3. 3′ and 5′ Rapid Amplification of cDNA Ends (RACE)
4.4. Data Availability
4.5. Gene Sequence Analyses
4.6. Deduced Amino Acid Sequence Analyses
4.7. Expression Analysis of pIgR Using Real-Time PCR
4.8. In Situ Hybridization (ISH)
4.8.1. Synthesis of RNA Probes
4.8.2. Staining Procedures
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Suborder | Species | pIgR Gene Size | 1st Intron | 2nd Intron | 3rd Intron | 4th Intron | 5th Intron | 6th Intron | 7th Intron |
---|---|---|---|---|---|---|---|---|---|
Notothenioidei | Trematomus bernacchii Gymnodraco acuticeps Pseudochaennichthys georgianus Cottoperca gobio | 8310 8235 9013 5930 | 628 583 582 596 | 2372 2866 3080 1519 | 548 552 538 204 | 2825 2906 2939 1713 | 124 124 133 156 | 146 85 85 129 | 111 111 111 115 |
Percoidei | Sander lucioperca Perca fluviatilis Perca flavescens Etheostoma spectabile Etheostoma cragini | 4818 3976 6669 5939 3700 | 649 590 554 579 595 | 124 126 125 124 124 | 211 211 211 153 207 | 1080 1237 1229 1014 985 | 161 171 171 143 143 | 155 137 156 137 145 | 115 122 115 115 117 |
Serranoidei | Epinephelus lanceolatus Plectropomus leopardus | 5831 7090 | 588 545 | 117 1304 | 206 183 | 2969 2135 | 163 161 | 93 150 | 107 118 |
Scorpaenoidei | Sebastes umbrosus | 6887 | 601 | 779 | 208 | 2631 | 634 | 133 | 118 |
Cottoidei | Pungitius pungitius Anarrhichthys ocellatus Gasterosteus aculeatus | 5126 5432 4095 | 579 595 577 | 461 593 274 | 196 205 195 | 872 1369 1210 | 136 176 89 | 167 153 159 | 96 106 99 |
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Ametrano, A.; Picchietti, S.; Guerra, L.; Giacomelli, S.; Oreste, U.; Coscia, M.R. Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei. Int. J. Mol. Sci. 2022, 23, 7783. https://doi.org/10.3390/ijms23147783
Ametrano A, Picchietti S, Guerra L, Giacomelli S, Oreste U, Coscia MR. Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei. International Journal of Molecular Sciences. 2022; 23(14):7783. https://doi.org/10.3390/ijms23147783
Chicago/Turabian StyleAmetrano, Alessia, Simona Picchietti, Laura Guerra, Stefano Giacomelli, Umberto Oreste, and Maria Rosaria Coscia. 2022. "Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei" International Journal of Molecular Sciences 23, no. 14: 7783. https://doi.org/10.3390/ijms23147783