Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin
Abstract
:1. Introduction
2. Results
2.1. Effects of Methylglyoxal-Induced Glycation of HSA
2.2. Parameters of Protein Interaction with Vaterite Microparticles
2.3. Neutrophil Activation by Soluble HSA-MG
2.4. Neutrophil Activation by Protein–Vaterite Microparticles
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Preparation of Methylglyoxal-Modified Albumin (HSA-MG)
4.3. The Molecular Mass of HSA and HSA-MG
4.4. Advanced Glycation Product (AGE) Detection
4.5. Two-Dimensional Difference Gel Electrophoresis (DIGE)
4.6. Scanning Electron Microscopy (SEM)
4.7. Fabrication of Vaterite Microparticles and Vaterite Microparticles with Co-Precipitated Protein
4.8. Light Microscopy of Vaterite Microparticles (CC)
4.9. Calcium Phosphate Nanowire-Composed Microparticles (CP) Preparation
4.10. Protein Adsorption onto CC and CP Particles
4.11. ζ-Potential Measurement
4.12. Blood Collection and Isolation of Neutrophils
4.13. Measurement of H2O2 Production by Human Neutrophils Based on Scopoletin Oxidation
4.14. Chemiluminescence Assay (CL)
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
Abbreviations
References
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Parameter | HSA | HSA-MG | p |
---|---|---|---|
Fluorescence, λex = 325 nm/λem = 430 nm | 0.31 ± 0.06 * | 0.41 ± 0.02 * | ˂0.001 |
Absorbance, λ = 320 nm | 0.016 ± 0.001 * | 1.376 ± 0.002 * | ˂0.001 |
ζ-potential, mV | −13.8 ± 2.2 | −15.9 ± 2.0 | |
MW, Da | 67,626 | 75,461 |
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Mikhalchik, E.V.; Ivanov, V.A.; Borodina, I.V.; Pobeguts, O.V.; Smirnov, I.P.; Gorudko, I.V.; Grigorieva, D.V.; Boychenko, O.P.; Moskalets, A.P.; Klinov, D.V.; et al. Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin. Int. J. Mol. Sci. 2022, 23, 7840. https://doi.org/10.3390/ijms23147840
Mikhalchik EV, Ivanov VA, Borodina IV, Pobeguts OV, Smirnov IP, Gorudko IV, Grigorieva DV, Boychenko OP, Moskalets AP, Klinov DV, et al. Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin. International Journal of Molecular Sciences. 2022; 23(14):7840. https://doi.org/10.3390/ijms23147840
Chicago/Turabian StyleMikhalchik, Elena V., Victor A. Ivanov, Irina V. Borodina, Olga V. Pobeguts, Igor P. Smirnov, Irina V. Gorudko, Daria V. Grigorieva, Olga P. Boychenko, Alexander P. Moskalets, Dmitry V. Klinov, and et al. 2022. "Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin" International Journal of Molecular Sciences 23, no. 14: 7840. https://doi.org/10.3390/ijms23147840