Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of MfWRKY7
2.2. Overexpressing MfWRKY7 in Arabidopsis Increased Drought and Salt Tolerance
2.3. MfWRKY7 Improved Osmotic Adjustment Ability in Transgenic Arabidopsis
2.4. Effect of MfWRKY7 on Antioxidant Metabolism in Arabidopsis under Drought and Salinity Stresses
2.5. MfWRKY7 Promoted Stomatal Closure Induced by Drought
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Cloning and Sequence Analysis of MfWRKY7
4.3. Vector Construction and Generation of Transgenic Lines
4.4. Phenotype Analysis under Drought and Salt Stresses
4.5. Measurement of Chlorophyll Content
4.6. Measurement of Water Loss Rate
4.7. Physiological Measurements
4.8. Stomatal Aperture Analysis
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Huang, Z.; Liu, L.; Jian, L.; Xu, W.; Wang, J.; Li, Y.; Jiang, C.-Z. Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 7890. https://doi.org/10.3390/ijms23147890
Huang Z, Liu L, Jian L, Xu W, Wang J, Li Y, Jiang C-Z. Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis. International Journal of Molecular Sciences. 2022; 23(14):7890. https://doi.org/10.3390/ijms23147890
Chicago/Turabian StyleHuang, Zhuo, Ling Liu, Linli Jian, Wenxin Xu, Jiatong Wang, Yaxuan Li, and Cai-Zhong Jiang. 2022. "Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis" International Journal of Molecular Sciences 23, no. 14: 7890. https://doi.org/10.3390/ijms23147890