Integrated Analysis of Microarray, Small RNA, and Degradome Datasets Uncovers the Role of MicroRNAs in Temperature-Sensitive Genic Male Sterility in Wheat
Abstract
:1. Introduction
2. Results
2.1. Pollen Development Was Defective in BS366 under Cold Conditions
2.2. Construction of the Co-Expression Network for All of the Expressed Genes
2.3. Expression and Function Analysis of Genes in the Blue and Brown Modules
2.4. The Differentially Expressed Genes between Cold and Control Conditions
2.5. Differential Expression of Genic Male Sterility Genes
2.6. Mapping of sRNA Reads to the Reference Genome
2.7. Differentially Expressed miRNA Identification, Target Prediction and Function Analysis
2.8. The Antagonistic miRNA–Target Pairs Identified during Three Stages
2.9. Identification of miRNA Target Genes via Degradome Sequencing
3. Discussion
3.1. The Potential Role of Conserved miRNAs in the Anther Development of Wheat
3.2. Impaired Cell Plate Formation during Meiosis
3.3. Suppression of Lipid Metabolism Was Responsible for Male Sterility in BS366
3.4. The Auxin Signaling Pathway Is Essential to Male Sterility in BS366
4. Materials and Methods
4.1. Plant Materials
4.2. Phenotypic Analysis of BS366
4.3. Data Acquisition
4.4. Bioinformatics Analysis
4.5. Weighted Gene Co-Expression Network Analysis
4.6. Sample Preparation, RNA Isolation, and Real-Time qRT–PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Y.; Li, D.; Zhang, S.; Zhang, L.; Gong, J.; Li, Y.; Chen, J.; Zhang, F.; Liao, X.; Chen, Z.; et al. Integrated Analysis of Microarray, Small RNA, and Degradome Datasets Uncovers the Role of MicroRNAs in Temperature-Sensitive Genic Male Sterility in Wheat. Int. J. Mol. Sci. 2022, 23, 8057. https://doi.org/10.3390/ijms23158057
Liu Y, Li D, Zhang S, Zhang L, Gong J, Li Y, Chen J, Zhang F, Liao X, Chen Z, et al. Integrated Analysis of Microarray, Small RNA, and Degradome Datasets Uncovers the Role of MicroRNAs in Temperature-Sensitive Genic Male Sterility in Wheat. International Journal of Molecular Sciences. 2022; 23(15):8057. https://doi.org/10.3390/ijms23158057
Chicago/Turabian StyleLiu, Yongjie, Dan Li, Shengquan Zhang, Liping Zhang, Jie Gong, Yanhong Li, Jiamin Chen, Fengting Zhang, Xiangzheng Liao, Zhaobo Chen, and et al. 2022. "Integrated Analysis of Microarray, Small RNA, and Degradome Datasets Uncovers the Role of MicroRNAs in Temperature-Sensitive Genic Male Sterility in Wheat" International Journal of Molecular Sciences 23, no. 15: 8057. https://doi.org/10.3390/ijms23158057