Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts
Abstract
:1. Introduction
2. Results
2.1. Morphological Features of 6-MITC and Its Derivatives on DCs
2.2. Effect of 6-MITC and Chemical Derivatives on DC Viability
2.3. Modulation by 6-MITC and Its Derivatives on DC Surface Marker Expression
2.4. Secretion of Cytokines from DCs in the Presence of 6-MITC and Its Derivatives
2.5. Effect of 6-MITC and I7557 on DC Stimulation of Allogeneic Naive T Cells
2.6. Effect of 6-MITC and I7557 on Allograft Survival
2.7. Histological Analysis
2.8. Immunohistochemical Study
2.9. Evaluation of Hepatotoxicity and Nephrotoxicity
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Generation of Dendritic Cells
4.3. Morphological Observation
4.4. Number of Viable Cells
4.5. Flow Cytometric Analysis
4.6. Allogeneic Naïve T-Cell Proliferation and Cytokine Secretion
4.7. Detection of Cytokines Produced by DC and Simulated Allogenic Naïve T cells
4.8. Animals
4.9. Skin Transplantation Model
4.10. Drug Preparation and Treatment
4.11. Histological Evaluation
4.12. Immunohistochemical Analysis
4.13. Evaluation of Leukocyte Count, Hepatic, and Renal Function
4.14. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Huang, T.-S.; Ko, C.-J.; Lin, J.-C.; Hsu, M.-L.; Ko, C.-C.; Chi, C.-W.; Tsai, T.-H.; Chen, Y.-J. Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts. Int. J. Mol. Sci. 2022, 23, 8488. https://doi.org/10.3390/ijms23158488
Huang T-S, Ko C-J, Lin J-C, Hsu M-L, Ko C-C, Chi C-W, Tsai T-H, Chen Y-J. Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts. International Journal of Molecular Sciences. 2022; 23(15):8488. https://doi.org/10.3390/ijms23158488
Chicago/Turabian StyleHuang, Tun-Sung, Chih-Jung Ko, Jiunn-Chang Lin, Ming-Ling Hsu, Chun-Chuan Ko, Chih-Wen Chi, Tung-Hu Tsai, and Yu-Jen Chen. 2022. "Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts" International Journal of Molecular Sciences 23, no. 15: 8488. https://doi.org/10.3390/ijms23158488